| 刘洁周国兴刘求梅徐筱红刘晓敏.胰岛素样生长因子1（IGF-I）通过PI-3K/Akt 途径对结肠癌细胞
凋亡的影响[J].现代生物医学进展英文版,2011,11(6):1078-1082. |
| 胰岛素样生长因子1（IGF-I）通过PI-3K/Akt 途径对结肠癌细胞
凋亡的影响 |
| Effect of Insulin-like Growth Factor-I on Apoptosis of Colon Cancer CellThrough Signaling Pathway of PI-3K/Akt |
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| DOI： |
| 中文关键词: IGF-I survivin p-Akt 凋亡 结肠癌 |
| 英文关键词: IGF-I survivin p-Akt apoptosis colon cancer |
| 基金项目:湖南省教育厅一般项目资助课题(08C037) |
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| 中文摘要: |
| 目的：探讨胰岛素样生长因子-1（IGF-I）通过磷酯酰肌醇3- 激酶/ 蛋白激酶B（PI-3K/Akt）信号通路对结肠癌细胞株SW480
凋亡率的影响及其凋亡抑制蛋白survivin 表达水平的变化。方法：培养结肠癌SW480 细胞株，实验分成三组：未加IGF-I 空白组、
IGF-I 刺激组、IGF-I+LY294002 阻断组，检测阻断剂LY294002 是否阻断PI-3K/Akt 通路（Western Blot 检测三组P-Akt 表达情
况）；Western Blot 及免疫荧光观察三组survivin 蛋白表达变化；MTT 法检测细胞增殖活性,流式细胞术检测细胞凋亡情况。结果：
Western blot 结果显示LY294002 可抑制IGF-I 诱导的p-Akt 的表达（P<0.05）；阻断IGF-I 诱导的PI-3K/Akt 通路后MTT 显示结肠
癌细胞SW480 增殖抑制率升高(P<0.05), 流式细胞术分析显示凋亡率明显上升(P < 0. 05) ；Western blot 及免疫荧光结果显示
LY294002 可抑制IGF-I 诱导的survivin 的表达（P<0.05）。结论：IGF-I 可通过PI-3K/Akt 通路诱导survivin 表达，从而抑制结肠癌
细胞SW480 的凋亡。 |
| 英文摘要: |
| Objective: To investigate the effect of IGF-I on the apoptosis rate，and the expression of survivin through the signaling
pathway of PI-3K/Akt in colon cancer cell line SW480. Methods: The experiment was divided into three groups: not-added IGF-I blank
group ,added IGF-I stimulate group,added IGF-I and the specific inhibitor LY294002 block group, detected the specific inhibitor
LY294002 could inhibited the signaling pathway of PI-3K/Akt(Western blot was used to detecte the expression of P-Akt of three groups);
Western blot and cell immunofluorescence were used to detecte the expression of survivin protein of three groups; SW480 cells proliferation
was measured by MTT assay. The apoptosis rate was analyzed by flow cytometry ( FCM). Result: When the signaling pathway of
PI-3K/Akt was inhibited by specific inhibitor LY294002, the expression of p-Akt and survivin proteins were obviously lower than the
IGF-I stimulate group (P<0.05); MTT showed that SW480 cells proliferation was significantly inhibited after the signaling pathway of
PI-3K/Akt was inhibited (P<0.05),The flow cytometry indicated that the apoptosis rate of SW480 cells could markedly upgrade(P<0.05）.
Conclusion: IGF-I could induce survivin expression through the PI-3K/Akt in colon cancer line SW480, then lead to the inhibition of its
apoptosis. |
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