| 杜衍晓1 杨竹丽2 达雨1 田臻1 王梦佳1 阎潇1 郑如松1 袁晓2.功能矫形前伸青春期大鼠下颌后浅层嚼肌细胞凋亡的研究[J].现代生物医学进展英文版,2011,11(6):1048-1051. |
| 功能矫形前伸青春期大鼠下颌后浅层嚼肌细胞凋亡的研究 |
| Superficial Masseter Muscle Cells Apoptosis after Function MandibularAdvancement in Juvenile Rats |
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| DOI： |
| 中文关键词: 功能矫形 浅层嚼肌 细胞凋亡 |
| 英文关键词: Functional orthopedics Superficial masseter muscles Apoptotic |
| 基金项目:国家自然科学基金（30770528）资助; 青岛市科技局科技支撑计划(10-3-3-4-4-nsh) |
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| 中文摘要: |
| 目的：研究功能矫形前伸大鼠下颌后浅层嚼肌细胞凋亡的变化规律，探讨功能矫形的肌肉改建机理。方法：选用50 只5 周
龄Sprague-Dawley(SD)雄性大白鼠，随机分为实验组和对照组各25 只。实验组大鼠戴自制上颌功能矫治嚣，引导下颌前伸，并打
开咬合。利用RT-PCR 方法检测两组大鼠浅层嚼肌Bcl-2 和Bax 基因表达情况，利用TUNEL 方法检测浅层嚼肌细胞凋亡情况。
结果：①Bcl-2 和Bax 基因表达随大鼠戴用矫治器时间的延长而升高，至第3 周开始下降但仍高于对照组，但Bax 的表达高于
Bcl-2。Bax/Bcl-2 比值随大鼠戴用矫治器时间的延长而升高，至第4 周开始下降。②TUNEL 实验结果显示浅层嚼肌细胞在戴用矫
治器1 天后，开始出现凋亡，随着时间延长而增加，至第3 周达到顶峰，第4 周开始下降。结论：①Bax/Bcl-2 比值升高促进浅层嚼
肌细胞凋亡。②功能矫形可引起浅层嚼肌细胞凋亡，导致肌肉的结构和功能发生适应性改建。 |
| 英文摘要: |
| Objective: To investigate the relationship of the apoptotic superficial masseter muscle cells after functional mandibular
advancement on juvenile rats. Methods: Fifty 5-week-old male Sprague-Dawley (SD) rats were randomly divided into experimental and
control groups. The mimic functional appliances were used in experimental groups, and the rats of two groups were killed on 1st, 7th,
14th, 21th, 28th day. The erminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick and labeling (TUNEL) and
Hoechst 33258method was used to detect superficial masseter muscle cells apoptosis. Bax and Bcl-2 expression was detected by PCR.
Results:①Bax and Bcl-2 expression and the value of Bax/Bcl-2 increased after insertion of the appliance, and reach the peak on the 21st
day then decreased. ② The apoptotic cells increased after insertion of the appliance, and reach the peak on the 21st day then decreased.
Conclusion: ① The increased value of Bax/Bcl-2 promoted superficial masseter muscle cells apoptosis. ② Superficial masseter muscles
cells appeared apoptosis and rebuilt after functional mandibular advancement. |
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