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目的：检测用阿霉素（doxorubicin DOXO）处理的骨髓瘤细胞株NCI-H929 中ATP 与自噬表达水平的变化，探讨两者之间的关联。方法：分别以DOXO 2umol/l 24h、DOXO 2umol/l 联用自噬抑制剂3MA 10mmol/l 24h 处理H-929 细胞后，采用MTT 法检测细胞存活率；ATP 生物发光法检测ATP 表达量；Western Blot 检测靶细胞自噬标志分子LC3 蛋白的表达。结果：各组相对未处理组存活率分别为54%、35%；相对未处理组%ATP 分别为400%、150%；DOXO 24h LC3 表达显著上调。结论：经DOXO 处理H-929 细胞系自噬形成,进而ATP 上升以保护细胞。

英文摘要:

Objective: As a major intracellular degradation system that is found ubiquitously in eukaryotes, autophagy plays a key role in maintaining protein metabolic equilibrium, cell homeostasis, regulating cellular constituent recycling, cell development and differentiation. In different cell types or different stress modes, the role or function of autophagy changes. What`s the function of autophagy in myeloma cells? We don't have experience at present. Based on the background above, in this study, by means of the model of myeloma cells (H-929 cells), we try to explore the effects of autophagy on cell death induced by DOXO and the correlating mechanism. Methods: After treated with DOXO (2umol/l for 24 h) with or without 3MA (10mmol/l), cells were collected and subjected to MTT assay, cellular ATP measurement and western blot for analysis. The ATP level was presented as percentage to the untreated control group. Results: The survival ratio was significantly higher in the DOXO group (54%) than that in the DOXO+3-MA group (35%); The ATP level changed (DOXO group 400%, DOXO+3-MA group 150%); Western blot showed that there was a high level of LC3II in DOXO singly treating cells and a low level in DOXO+3-MA group. Conclusions: 1. Autophagy as protective mechanism responses to the death induced by DOXO; 2. Autophagy inhibition by chemical inhibitor enhances the death induced by DOXO in myeloma cell lines; 3. It has a relation with ATP.

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