| 达雨1 杨竹丽2 袁晓2△ 杜衍晓1 田臻1 王梦佳1.功能矫形前伸青春期大鼠下颌后翼外肌MyoD、myogenin 的表达[J].现代生物医学进展英文版,2011,11(6):1020-1024. |
| 功能矫形前伸青春期大鼠下颌后翼外肌MyoD、myogenin 的表达 |
| Expression of MyoD, Myogenin mRNA in Juvenile Rats LateralPterygoid after Functional Mandibular Protrusion |
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| DOI： |
| 中文关键词: 应力介导：成肌细胞：分化：成肌调节因子：翼外肌 |
| 英文关键词: Stress-induced Myoblast Differentiation MRFs Lateral pterygoid |
| 基金项目:国家自然科学基金资助项目（30871426, 30770528）青岛市科技局科技支撑计划(10-3-3-4-4-nsh) |
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| 中文摘要: |
| 目的：探讨" 应力- 生长（改建）" 在细胞水平上的体现，为功能矫形治疗和矫治效果的保持提供新思路和实验依据。方法：本
实验选用20 只4 周龄，雄性SD 大鼠随机分为8 组。其中实验组大鼠经戊巴比妥麻醉后佩戴上颌斜面导板，对照组未佩用。依据
时间不同又分为四组：1d，7d，14d，21d。采用RT-PCR 技术分析各组大鼠翼外肌组织中肌分化相关基因MyoD、myogenin mRNA
的表达变化。结果：未施加功能矫形力的大鼠翼外肌组织MyoD 表达伴随其生长发育呈现递减趋势，实验组在第7 d 出现表达上
调。同时，力学刺激后实验组动物myogenin 的表达与对照组相比较在14 d 组出现明显上调。结论：功能矫形力作用于翼外肌组织
可以诱导MyoD 和myogenin 的表达上调进而诱导成肌细胞的分化。 |
| 英文摘要: |
| Objective: To investigate the expression of "stress - growth (reconstruction) " at the cellular level for provide new ideas
and experimental evidence about functional orthopedic treatment and treatment effects remain. Methods: Twenty, 28 days old, male
Sprague-Dawley (SD) rats were chosen in this research. SD rats are randomly divided into eight groups: control 1 d, 7 d, 14 d, 21 d and
experimental 1 d, 7 d, 14 d, 21 d. When rats were anaesthetized by intraperitoneal injections of pentobarbital, the inclined bite plate were
put into its mouth. RT-PCR was taken at 1, 7, 14, 21 days after appliance activation. The gene expression of lateral pterygoid MyoD and
myogenin mRNA is determined by RT-PCR technology. Results: MyoD, myogenin mRNA decreased in all juvenile rats lateral pterygoid.
And expression of MyoD in control group reduced, while myogenin enhanced in 7 d. With the removable functional appliance-inclined
bite plate rats, both the expressions of MyoD and myogenin enhanced, especially in 7 d. Conclusions: Expression of MyoD and myogenin
mRNA in juvenile rats lateral pterygoid after functional mandibular protrusion increased. |
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