| 周后龙1 巩丽1 李艳红3 刘小艳2 兰淼2 张贺龙1 张伟2△ 冯英明1△.原发性肝癌染色体8p、16q 遗传变异的研究[J].现代生物医学进展英文版,2011,11(5):844-849. |
| 原发性肝癌染色体8p、16q 遗传变异的研究 |
| Study on Genetic Alterations in Chromosomes 8p and 16qin Primary Hepatocellular Carcinoma |
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| DOI： |
| 中文关键词: 遗传变异 杂合性丢失 微卫星不稳定 肝细胞肝癌 染色体 |
| 英文关键词: Genetic alterations Loss of heterozygosity Microsatellite instability Human hepatocellular carcinoma Chromosome |
| 基金项目:国家自然科学基金资助项目：No.30800147；No.30672013 |
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| 中文摘要: |
| 目的：分析人肝癌（HCC）组织中染色体8p、16q 部分基因及染色体片段的遗传变异及与临床病理关系，初步筛选HCC 相关
的抑癌基因。方法：应用聚合酶链反应- 变性聚丙烯酰胺凝胶- 银染法分析45 例HCC 组织标本中染色体8p 和16q 的杂合性丢
失（LOH） 及微卫星不稳定性（MSI）。结果：发生LOH 的总频率为68.89%(31/45)，其中D16S511 位点的发生LOH 率最高为
53.33%(24/45)，其次是D8S261（39.02%，16/41）和D8S499（34.88%，15/43）。MSI 出现的总频率为11.11%（5/45），出现在三个微卫
星位点（D8S261、D8S499 及D16S511）上。结论：染色体16q23、8p22-21.3 及8p12 区域的LOH 发生频率高，其可能存在与HCC
发生发展相关的新的抑癌基因，特定位点的遗传变异可能与HBV 感染、临床病理恶性程度等预后因素相关。 |
| 英文摘要: |
| Objective: To investigate the genetical alterations on chromosomes 8p and 16q in primary hepatocellular carcinoma
(HCC), investigate the relationship between the gentical alterations and clinicopathologic features and try to screened some HCC-related
tumor suppressor genes. Methods: Loss of heterozygosity (LOH) and microsatellite instability (MSI) on chromosome 8p and 16q in
samples from thirty-five patients with HCC were examined by PCR-denaturing PAGE-silver staining. Results: The overall LOH
frequency was 68.89%(31/45) at least one locus of 8 loci on the chromosomes. The frequency of Loci were 53.33% (31/45),39.02%
（16/41）and34.88%（15/43）for D16S511, D8S261 and D8S499, respectively. The frequency of MSI was 11.11%(5/45) and the MSI was
distributed on the three microsatellite markers (D16S511、D8S261 and D8S499). Conclusion: There may be a new putative tumor
suppressor gene related to the occurrence and development on specific chromosome region 16q23, 8p22-21.3 or 8p12 with high-frequent
LOH. The genetic alterations on some specific loci were associated with prognosis factors as the positive HBsAg, differentiated degrees
of HCC. |
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