| 吴建红邵怡刘海涛陈辉容洪艳夏术阶△.糖尿病大鼠膀胱和骶髓背根神经节中NGF 的表达与尿流动力学
改变的研究[J].现代生物医学进展英文版,2011,11(5):824-826. |
| 糖尿病大鼠膀胱和骶髓背根神经节中NGF 的表达与尿流动力学
改变的研究 |
| Expression of NGF in lumbar dorsal root ganglia and bladder of diabeticrats and urodynamic changes |
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| DOI： |
| 中文关键词: 糖尿病膀胱 神经生长因子 尿流动力学 |
| 英文关键词: Diabetic cystopathy nerve growth factor(NGF) Urodynamics |
| 基金项目:上海市科学技术委员会科研计划项目资助(074119518) |
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| 中文摘要: |
| 目的：探讨糖尿病大鼠膀胱与骶髓背根神经节（DRG）中神经生长因子（NGF）的表达与尿流动力学改变。方法：建立糖尿病
大鼠模型10 只，对照组10 只，应用酶联免疫吸附试验（ELISA）法，分别检测大鼠膀胱组织及骶髓DRG 中NGF 的变化情况，结合
代谢笼及尿流动力学改变，探讨糖尿病膀胱病变的可能发病机制。结果：造模12 周后，糖尿病大鼠膀胱容量较正常对照组明显增
大（1.47±0.28vs0.71±0.12,p<0.05），残余尿量明显增多(0.52±0.18vs0.07±0.08，p<0.01)，排尿效率明显下降，膀胱及骶髓DRG 中
NGF 表达水平明显降低。结论：NGF 在糖尿病大鼠膀胱和骶髓背根神经节中低表达，在糖尿病膀胱病变中发挥着重要作用。 |
| 英文摘要: |
| Objective: To evaluate the expression of nerve growth factor (NGF) in the bladder and sacral cord dorsal root ganglia
(DRG) and the bladder function 8 weeks after diabetes induction and investigate the pathogenesis of diabetic cystopathy. Methods:
Twenty Sprague-Dawley rats were divided into two groups: control (n = 10), streptozotocin-induced diabetic group (n = 10). Eight weeks
later, the bladders and sacral cord DRG were dissected. We measured the expression of NGF in the bladders and sacral cord DRG using
ELISA, evaluated the bladder function by metabolic cage study and cystometry. Results: We found that the streptozotocin-induced
diabetic rats showed impaired bladder function characterized by increased bladder capacity, decreased bladder contractility (voiding
efficiency), and an increase in residual urine. We also found a significantly reduced expression of NGF in the bladders sacral cord DRG
from the diabetic group compared with the control. Conclusions: Our findings indicated that the decrease in NGF may be a contributory
factor in diabetic cystopathy. |
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