| 李英卓张志培范崇熙汪健倪云峰邓迎春杨菁李小飞△ 程庆书.连续观察兔油酸ARDS模型稳定性及血L-1、IL-8近期变化[J].现代生物医学进展英文版,2011,11(4):637-641. |
| 连续观察兔油酸ARDS模型稳定性及血L-1、IL-8近期变化 |
| The Stability and Changes of IL-1, IL-10 in Plasma of New ZealandRabbits with Acute Respiratory Distress Syndrome |
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| DOI： |
| 中文关键词: 油酸 呼吸窘迫综合征ARDS IL-1 IL-8 兔 |
| 英文关键词: Oleic acid ARDS IL-1 IL-8 Rabbit |
| 基金项目:全军医药卫生科研项目（2008080202） |
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| 中文摘要: |
| 目的：探讨油酸（OA）构建兔急性呼吸窘迫综合征（ARDS）模型3 天内的稳定性及血浆炎症因子IL-1、IL-8 含量变化的意
义。方法：健康新西兰大耳白兔30 只随机分为5 组，每组6 只，实验组（n=24）耳缘静脉注射油酸（0.1mL/kg）建立ARDS模型，对
照组（n=6）注射等量生理盐水。分别检测对照组6h 和实验组6h、24h、48h、72h（n=6）动脉血PH 值、PaO2、PaO2/FiO2、PaCO2、肺湿/
干重比值（W/D），ELISA法检测血浆细胞因子IL-1、IL-8含量，HE染色观察肺组织病理学改变。结果：和对照组比较，实验组血中
细胞因子IL-1在6h、24h组升高；细胞因子IL-8在6h组、24h组、48h组升高。6h 组、24h 组、48h组氧合指数<200mmHg。结论：兔
油酸ARDS模型48h内稳定，炎性细胞和IL-1、IL-8可能是导致ARDS的发生和发展主要原因之一。 |
| 英文摘要: |
| Objective: To investigate the stability and the changes of IL-1, IL-10 in plasma of New Zealand rabbits with acute respiratory
distress syndrome (ARDS) induced by oleic acid (OA) in 3 days. Methods: Thirty New Zealand rabbits were randomly divided
into OA experimental groups which were injected with oleic acid (0.1mL/kg) via ear vein and control group which was injected with normal
saline (0.1mL/kg). Histopathology, wet weight/dry weight ratio (W/D) of the lung, arterial plasma PH, PaO2, PaCO2 were observed at
6h, 24h, 48h 72h groups after injection. ELISA method was used to determine plasma IL-1 and IL-8. Results: Compared with control
group, the levels of PO2 of experimental groups decreased (p<0.01) while the levels of PaCO2 of experimental groups increased (p<0.01).
Compared with the 72h group, the levels of PO2 of other experimental groups decreased (p<0.01) while the levels of PaCO2 of 24h, 48h
groups increased (p<0.01). The wet weight/dry weight ratios(W/D) of the lung increased in 6h, 24h, 48h groups (p<0.01) compared with
control group while they decreased in 48h and 72h groups (p<0.01) compared with that of the 6h group. The levels of IL-1 in plasma
were higher in 6h group (p<0.01) and in 24h group (p<0.05) than that in control group, while which were lower in 48h and 72h groups
(p<0.01) than that in 6h group. And the levels of IL-8 in plasma were higher in 6h, 24h, 48h groups (p<0.01) than that in control group
while which were lower in 24h, 48h groups (p<0.05) and in 72h group (p<0.01) than that in 6h group. The 6h, 24h, 48h groups could be
diagnosed with ARDS by PaO2/FiO2<200mmHg but the 72h group's PaO2/FiO2>200mmHg. Conclusion: The ARDS model set up by oleic
acid is stable in 48h, and the he inflammation cells and increase of IL-1, IL-8 might play important roles in ARDS. |
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