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目的：研究β2 肾上腺素能激动剂福莫特罗（Formoterol）对大鼠的体外骨髓间充质干细胞（MMSC）向成骨细胞分化的影响，进而探讨其作用机制。方法：取SD大鼠的骨髓间充质干细胞，用条件培养液诱导分化后分别加入不同浓度药物，在不同时间点采用RT-PCR法检测细胞分化中Runx2 和Osterix 的mRNA的表达，采用western blot法检测细胞中MEK和ERK1/2 的磷酸化。结果：在细胞分化早期，加入已知浓度10-7mol/L 的Formoterol 可抑制成骨样细胞细胞特异性转录因子Runx2 mRNA 表达；在细胞分化晚期，浓度10-7mol/L的Formoterol也可抑制成骨样细胞Osterix mRNA 表达。在加入浓度10-7mol/ 的Formoterol 作用30min 后，MEK和ERK1/2 的蛋白磷酸化表达均下降。结论：β2 受体激动剂可抑制MMSC细胞体外向成骨样细胞的分化，并且可抑制 MEK和ERK1/2 磷酸化的表达。

英文摘要:

Objective: To observe the effects of Formoterol（β2-adrenergic receptor-specific agonist）on the differentiation of rat osteoblast-like cells. Methods: Osterix and RUNX2 are osteoblast-specific transcriptional factors that are essential for osteoblast differentiation and bone formation. RT-PCR was used to detect the Osterix and RUNX2 genes expressions during the treatment of different drug dose,the expression of P-MEK and P-ERK1/2 were measured by western blot. Results: ①The mRNA expression of cells cultured with a concentration of 10-7mol/L of Formoterol are obviously low. ②The protein phosphorylation of ERK and MEK in osteroblasts decreased at 30min treating the cells with Formoterol. Conclusions: In the present study, we examined the molecular mechanism of Osterix and RUNX2 gene expression, and found that Formoterol inhibited Osterix and RUNX2 expression in the mouse osteoblast-like cells, Formoterol-induced decrease of Osterix and RUNX2 expression may occurred in dose-dependent manner through the activation of extracellular signal-regulated kinase pathway.

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