| 刘靓1 胡学昱1 张蕾2 尤凤健1 黄景辉1 罗卓荆1.高仿真组织工程神经支架制备工艺的参数优化[J].现代生物医学进展英文版,2011,11(4):624-627. |
| 高仿真组织工程神经支架制备工艺的参数优化 |
| Parameter Optimization of the Fabrication Technique for Highly BionicTissue Engineering Nerve Scaffold |
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| DOI： |
| 中文关键词: 仿真神经支架 结构特征 孔径 醋酸 冷淋速度 |
| 英文关键词: Bionic nerve scaffold Micro-structural characteristic Pore size Acetic acid Freezing velocity |
| 基金项目:国家自然科学基金资助项目(30770571和30973052) |
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| 中文摘要: |
| 目的：对直接影响神经支架微观结构的关键因素进行分析，以确定制备不同孔径仿真支架的制备工艺。方法：用前期开发的
神经支架制备工艺，应用不同浓度的醋酸浓度和冷淋速度制备仿真神经支架，以扫描电镜观察神经支架结构特征，以确定醋酸浓
度和冷淋速度对神经支架内部结构的影响。结果：醋酸浓度和冷淋速度对神经支架内部结构具有重要影响。醋酸浓度为0mg/ml
时，无法制备定向结构的神经支架，当醋酸浓度为1 mg/ml、2 mg/ml、3 mg/ml 和4 mg/ml 时，可制备轴定向仿真支架，并且神经支
架的孔径随醋酸浓度增大而增大；当冷淋速度为1×10-5m/s、2×10-5m/s 和5×10-5m/s 时，所制备的仿真支架内部均呈明显的轴向
微管结构，其中冷淋速度为2×10-5m/s时，其轴向微管结构排列最为有序、规律。当速度为1×10-6m/s，2×10-6m/s，5×10-6m/s 以及
1×10-4m/s时，所制备的材料内部微管结构走向无明显规律。结论：醋酸浓度和冷淋速度是影响神经支架内部结构的两个关键因
素，通过改变醋酸浓度和冷淋速度可制备不同孔径的仿真神经支架。 |
| 英文摘要: |
| Objective: To investigate the factors which influence the micro-structural characteristics of the highly bionic nerve
scaffold, and to determine the technology for establishing bionic nerve scaffold with different micro-structural characteristics. Methods:
Nerve scaffolds with different micro-structural characteristics were fabricated with varied doses of acetic acid and a series of freezing
velocities. Scanning electron microscopy was used to detect the micro-structural characteristics. The effects of acetic acid concentration
and freezing velocities on the structural appearance of the scaffolds were determined. Results: The concentration of the acetic acid and
freezing velocities had effect on the micro-structural characteristics of the nerve scaffold. Scaffold with longitudinal pores could be fabricated
in the presence of acetic acid (1 mg/ml, 2 mg/ml, 3 mg/ml and 4 mg/ml, except 0mg/ml), and the pore size increased with
the increasing of the acetic acid concentration. In addition, highly bionic nerve scaffold could be made when the freezing velocities were
maintained at 1×10-5m/s, 2×10-5m/s and 5×10-5m/s, of which the most homogeneous longitudinal pores were achieved by velocity at
2×10-5m/s. When the velocity was maintained at 1×10-6m/s, 2×10-6m/s, 5×10-6m/s and 1×10-4m/s, the scaffold showed no longitudinal
pores. Conclusion: Acetic acid concentration and freezing velocities were two key factors determining the micro-structural characteristics
of the highly bionic nerve scaffold. Scaffolds with different micro-structural appearances can be fabricated through changing the acetic
acid concentration and freezing velocities. |
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