贾济1 朱萧玲1 左志义2 熊利泽1 陈绍洋1.CBR2激活与小胶质细胞的活化和损伤的关系[J].现代生物医学进展英文版,2011,11(4):601-604. |
CBR2激活与小胶质细胞的活化和损伤的关系 |
The relationship between CBR2 Activation and Injury Inducedby Inflammation |
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DOI: |
中文关键词: CBR2 小胶质细胞 炎症 活化 损伤 |
英文关键词: CB2 Microglia Inflammation Activation Injury |
基金项目:国家自然科学基金资助课题(81050032);海外及港澳学者合作研究基金(81028006) |
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中文摘要: |
目的:探讨大麻素CBR2 受体激动剂AM1241预处理对脂多糖(LPS)和γ-干扰素(IFN-γ)所致炎症反应对小胶质细胞活化
和损伤的影响。方法:联用LPS 和IFN-γ 作为小胶质细胞损伤模型,将细胞分为Control 组、AM1241 组、LPS/IFN-γ 组和
AM1241+ LPS/IFN-γ 组;AM1241 组和AM1241+ LPS/IFN-γ 组经AM1241 预处理2h,LPS/IFN-γ 组和AM1241+ LPS/IFN-γ
组用含LPS和IFN-γ 的培养基培养24h。采用MTT法检测细胞代谢率,硝酸还原酶法检测细胞培养液中一氧化氮(NO)释放量,
酶联免疫吸附剂测定细胞培养基中炎症因子释放量,倒置相差显微镜观察细胞形态。结果:与LPS/IFN-γ 组相比,AM1241+
LPS/IFN-γ 组细胞代谢率明显升高(P<0.05),NO、TNF-α、IL-1β 和IL-10 释放量明显减少(P<0.05),活化和损伤程度明显减
轻。结论:大麻素CBR2 受体激动剂AM1241预处理可减轻LPS和IFN-γ 对小胶质细胞的活化和损伤。 |
英文摘要: |
Objective: To investigate the effects of cannabinoid CBR2 receptor agonist AM1241 preconditioning on microglia
activation and injury induced by lipopolysaccharide (LPS) plus interferon-γ (IFN-γ). Methods: LPS plus IFN-γ was used to induce
inflammation. MTT assay was used to find a suitable AM1241 concentration for preconditioning. Cells were pretreated with medium
containing different AM1241 concentrations, from 0μM to 10μM; 5μM was chosen for next steps. Then cells were assigned to control
group, AM1241 group, LPS/IFN-γ group and AM1241+LPS/IFN-γ group. After AM1241 pretreatment, the medium of 4 groups were
changed with normal medium. 2h later, the medium of Control and AM1241 groups were changed with normal medium again and cultured
for 24h. The medium of LPS/IFN-γ and AM1241+ LPS/IFN-γ groups were changed with medium containing LPS and IFN-γ.
Cells were cultured for 24h. Microglial metabolism was assessed by MTT assay; NO release was measured by Reagent Kit; The concentrations
of inflammatory factors (TNF-α, IL-1β and IL-10) were detected by enzyme linked immunosorbent assay reagent kit (ELISA);
Microglial shapes were observed through microscope. Results: cell metabolism of AM1241 group was higher than that of the Control
group significantly (P<0.05); AM1241 group released less NO, TNF-α, IL-1β and IL-10 than that of LPS/IFN-γ group (P<0.05).
Conclusion: Cannabinoid CBR2 receptor agonist AM1241 preconditioning reduces microglial activation and injury induced by inflammation. |
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