| 吕沁风吴忠华郑伟徐琦孟军李禾.不同加热方式对嗜肺军团菌环介导等温扩增检测法的影响[J].现代生物医学进展英文版,2011,11(3):493-496. |
| 不同加热方式对嗜肺军团菌环介导等温扩增检测法的影响 |
| Effect of Different Heating Methods on Legionella PneumophilaLoop-mediated Isothermal Amplification Detection |
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| DOI： |
| 中文关键词: 环介导等温检测 加热方式 嗜肺军团菌 水浴 |
| 英文关键词: Loop-mediated isothermal amplification (LAMP) Heating method Legionella pneumophila Water bath |
| 基金项目:浙江出入境检验检疫局科技计划项目（ZK2008012） |
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| 中文摘要: |
| 目的：研究不同的加热方式对嗜肺军团菌环介导等温扩增检测法的影响方法：用已知的13 株嗜肺军团菌样本，采用空气
浴、水浴和PCR仪同时进行环介导等温扩增，观察沉淀反应、荧光反应以及产物电泳结果。结果：水浴和PCR仪加热LAMP反应
的沉淀产物较多，荧光反应较强，电泳检测结果较为明显。空气浴的3 种检测结果均较弱。结论：采用水浴和PCR仪进行环介导
等温扩增反应的效果较好，从仪器设备的成本及实验条件考虑，采用水浴是环介导等温扩增反应首选的加热方式。 |
| 英文摘要: |
| Objective: To investigate the effect of the three heating methods on loop-mediated isothermal amplification(LAMP)
detection of Legionella pneumophila.. Methods: DNA of 13 strains of Legionella pneumophila were amplified by LAMP based on air
bath, water bath, and PCR instrument. The results were observed by precipitation, fluorescent reaction and electrophoresis. Results: The
product of LAMP based on water-bath-heating method and PCR-instrument-heating presented more obvious precipitation, stronger fluorescence
reaction, lighter gel electrophoresis. However, the air-bath-heating method showed much more weak results. Conclusion: The
LAMP amplifications performed by water-bath-heating and PCR-instrument-heating, water bath heating were more economical choice
for LAMP. Considering the cost of equipment and experimental conditions, water-bath was the preferred heating method. |
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