| 戚大石1 汤佳立2.不同染色技术和双色荧光原位杂交技术对宽翅菘蓝
（Isatis violascens Bunge）的细胞遗传学分析[J].现代生物医学进展英文版,2011,11(3):485-488. |
| 不同染色技术和双色荧光原位杂交技术对宽翅菘蓝
（Isatis violascens Bunge）的细胞遗传学分析 |
| Cytogenetic Analysis of Isatis Violascens Bunge by Different StainingTechniques and D-Fluorescent in Situ Hybridization |
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| DOI： |
| 中文关键词: 宽翅菘蓝 银染 45S rDNA 双色荧光原位杂交 CMA3染色 |
| 英文关键词: Isatis violascens Bunge Silver staining 45S rDNA D-fluorescence in situ hybridization CMA3 staining |
| 基金项目: |
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| 中文摘要: |
| 为了解十字花科菘蓝属植物宽翅菘蓝（宽翅菘蓝，Isatis violascens Bunge）的染色体结构，本文利用45S rDNA 和5S rDNA 双
色荧光原位杂交、银染技术和CMA3 对宽翅菘蓝进行分子细胞遗传学研究。45S rDNA 和5S rDNA 荧光原位杂交结果显示宽翅
菘蓝染色体上有1对45S rDNA 信号和1 对5S rDNA 信号；银染结果显示其中期染色体有1 对银染点；CMA3 染色结果发现宽
翅菘蓝中期染色体存在1对CMA3信号。 |
| 英文摘要: |
| Objective In order to understand the chromosome structure of I. violascens Bunge, 45S rDNA and 5S rDNA dual-fluorescence
in situ hybridization (45S rDNA and 5S rDNA D-FISH), silver staining and CAM3 staining were used to study the chromosomes
of I. violascens Bunge. In the silver stained metaphase chromosomes of I. violascens Bunge, there were two positive regions; fluorochrome
Chromomycin A3 (CMA) staining showed two positive regions; D-FISH with 45S rDNA and 5S rDNA probes revealed one
18S-5.8S-26S rDNA loci and one 5S rDNA loci with different intensity. |
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