胡超△ 黄丽华左斌谢达平.酵母产γ-氨基丁酸发酵培养基的优化[J].现代生物医学进展英文版,2011,11(3):482-484. |
酵母产γ-氨基丁酸发酵培养基的优化 |
Optimization of Culture Medium for Gamma-aminobutyricAcid Producedby Saccharomyces Cerevisiae Strain |
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DOI: |
中文关键词: γ-氨基丁酸 酵母 培养基优化 |
英文关键词: Gamma-aminobutyric acid Saccharomyces cerevisiae Culture medium optimization |
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中文摘要: |
目的:提高酵母产γ- 氨基丁酸的能力。方法:采用单因素及正交设计实验对酵母产γ- 氨基丁酸(GABA)的培养基进行优
化。结果:确定最适碳源为葡萄糖,最佳氮源为蛋白胨和硫酸铵复合氮源,合适的无机盐为KH2PO4;最佳发酵培养基为3%葡萄
糖,3%蛋白胨,0.3%(NH4)2SO4和0.1%KH2PO4。在此培养条件下,摇瓶发酵可以获得1.690 g.L-1的GABA产量。结论:发酵培养基
的优化,提高了菌株产γ-氨基丁酸的能力。 |
英文摘要: |
Objective: To improve Gamma-aminobutyric acid (GABA) production by Saccharomyces cerevisiae. Methods: The
culture medium was researched by single factor and orthogonal experiment. Results: The optimum carbon and nitrogen sources were
glucose and the mixture of peptone and (NH4)2SO4, the optimal inorganic salt was KH2PO4. The preferable medium was as follows: 3%
glucose,3% peptone, 0.3% (NH4)2SO4, and 0.1% KH2PO4. Under the optimum conditions, the content of GABA reached 1.690 g.L-1.
Conclusion: The production of GABA was increased under the optimal conditions. |
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