| 练光辉1,2 陈旻湖1△ 彭侠彪2 阮巍山2 叶建明2.嗜酸乳杆菌胞壁粗提物及DNA对实验性结肠炎结肠上皮NF-κB
表达的影响[J].现代生物医学进展英文版,2011,11(3):468-471. |
| 嗜酸乳杆菌胞壁粗提物及DNA对实验性结肠炎结肠上皮NF-κB
表达的影响 |
| Effects of L. Acidophilus BCW and DNA on Expression of NF-κBin Murine with Acute Ulcerative Colitis |
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| DOI： |
| 中文关键词: 溃疡性结肠炎 乳酸杆菌 胞壁成分 DNA 细胞核因子-κB |
| 英文关键词: Ulcerative colitis Lactobacillus Bacterial cell walls DNA NF-κB |
| 基金项目:中山市科技局资助项目（20091A060） |
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| 中文摘要: |
| 目的：研究嗜酸乳杆菌BCW和DNA对溃疡性结肠炎小鼠结肠粘膜上皮NF-κB表达的影响。方法：雌性BABL/c 小鼠40
只随机分为正常组、嗜酸乳杆菌BCW组、嗜酸乳杆菌DNA 组和生理盐水对照组，除正常组小鼠外，其余各组自由饮用1.5%DSS
7天建立小鼠结肠炎模型，随即给予嗜酸乳杆菌BCW（20ug/10g）、嗜酸乳杆菌DNA（0.2ug/10g）和生理盐水灌肠7 天，每天观察小
鼠情况，实验结束时处死小鼠，去结肠组织行HE染色观测结肠炎症情况，并行结肠上皮NF-κB免疫组化。结果：饮用DSS小鼠
DAI积分显著增高，结肠组织粘膜破坏、炎症细胞浸润，结肠上皮NF-κB 表达增加（9.15±0.43），嗜酸乳杆菌BCW和DNA 能降
低小鼠DAI积分，减轻粘膜损伤，降低结肠上皮NF-κB的表达（4.67±0.56/6.03±0.60）。结论：嗜酸乳杆菌BCW和DNA能缓解
急性溃疡性结肠炎，降低结肠上皮NF-κB的表达。 |
| 英文摘要: |
| Objective: To investigate the effect of L. acidophilus BCW and DNA on the expression of NF-κB in murine intestinal
mucosa with UC. Methods: 40 specific pathogen free, BALB/c female mice were randomly divided into acute normal group, L. acidophilus
BCW-treated group, L. acidophilu DNA-treated group and NS control group. Mice were induced by 1.5% DSS water for 7 days
free drinking except nomal group. The mice were respectively received L. acidophilus BCW (20ug/10g), L. acidophilu DNA(0.2ug/10g)
and physiological saline, while mice of normal control group were untreated. The animal were recorded daily and killed after 7days for
assaying the NF-κB with immunohistochemical staining. Results: Both DAI scores and leisions of colonic mucosa of DSS-induced mice
decreased markedly by L. acidophilus BCW and it's DNA, while they were more severe than that of normal. Compared with the model
control group (9.15±0.43), the expression of NF-κB decreased significantly (BCW:4.67±0.56,DNA:6.03±0.60), but which was still
higher than that of normal. Conclusion: The L. acidophilus BCW and it's DNA alleviate DSS-induced ulcerative colitis and decreased the
expression of NF-κB in intestinal mucosa. |
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