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目的：探讨阿司匹林对骨髓基质细胞成骨性分化的影响。方法：培养SD大鼠骨髓基质细胞（BMSCs）, 传代3 次后进行成骨诱导分化，诱导培养基中加入不同浓度阿司匹林（0.5、1、2、5、10mmol/L），同时设立对照组。采用cck-8 法分析细胞增殖情况。比较阿司匹林组与对照组在细胞碱性磷酸酶（ALP）活性、骨钙素（OC）分泌量、钙结节染色等方面的成骨性差异。结果：阿司匹林无促进细胞增殖活性，而高浓度阿司匹林能够强烈抑制细胞增殖。0.5、1、2 mmol/L浓度阿司匹林可促进BMSCs 的成骨性分化，中低浓度组碱性磷酸酶含量、骨钙素分泌量在不同阶段显著高于对照组。14天茜素红染色可见中低浓度组钙结节数量高于对照组。结论：中低浓度阿司匹林作用于骨髓基质细胞可促进其成骨细胞特性表达,这表明阿司匹林有促进骨代谢合成的作用。

英文摘要:

Objective: To investigate the effect of aspirin on differentiation of marrow stromal cells (MSCs) into osteoblast in vitro. Methods: Bone marrow stromal cells of 7 days old SD rats were cultured. After 3 times of passages, the cells were induced to osteogenic differentiate in mediums that contained 0.5, 1, 2, 5,10mmol/L aspirin separately, and control group was establish. The cell proliferation was assayed by CCK-8 method. The osteogenic differentiation markers including alkaline phosphatase (ALP) activity, osteocalcin secretion and mineralized bone modulus between the Aspirin groups and the control were compared. Results: Aspirin didn't stimulate the proliferation of BMSCs, and high concentration of aspirin had the inhibited effect. 0.5, 1, 2 mmol/L aspirin stimulated the osteogenic differentiation of BMSCs. In different phase, alkaline phosphatase activity and osteocalcin secretory volume in low and medium concentration of aspirin groups was higher than that of the control group. Alizarin red stain for calcium nodus in aspirin groups was far more than that in control group in 14 days. Conclusions: Low and medium concentration of aspirin stimulated the osteogenic differentiation of BMSCs, which indicated that aspirin had the ability to enhance the bone metabolism activity.

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