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目的：探讨疏肝补肾法对疲劳大鼠学习和记忆力及对海马CA1 区神经颗粒素(Neurogranin, Ng) 的mRNA 表达变化的影响。方法：成年雄性Spargue-Dawley大鼠36只，随机分为模型组(MG)、对照组(CG)、和疏肝补肾组(LK)。采用复合模型：运动疲劳模型与睡眠剥夺法造疲劳大鼠模型。运用Y迷宫进行学习和记忆力的测试。以Real-time PCR 技术分析海马CA1区神经颗粒素的mRNA表达。结果：Y迷宫实验显示用药后大鼠的学习和记忆能力优于模型组，而疏肝补肾组大鼠在正确反应率、错误反应次数、达标所需训练次数和总反应时间皆与模型组有差异(分别为P<0.01、P<0.01、P<0.05 和P<0.05)，其Ng mRNA 在海马CA1 区的表达也显着高于模型组(P<0.01)。结论：复合模型会造成大鼠学习和记忆能力受损。疏肝补肾法能显着影响疲劳大鼠的学习记忆能力及海马CA1区Ng的mRNA表达。

英文摘要:

Objective: To study the effects of regulating liver Qi and tonifying kidney on learning and memory of fatigue rats, and on the mRNA level of neurogranin in the CA1 region of rats hippocampus. Methods: Thirty-six male adult Spargue-Dawley rats were randomly separate into three groups, model group (MG, n=12), control group (CG, n=12) and regulate liver Qi and tonify kidney group (LK, n=12). By using combined models: exercise fatigue model and sleep deprivation as our fatigue model. Y maze has been used to test the memory and learning of rats and the mRNA level of Neurogranin was tested by using Real-time PCR. Results: The results from Y maze test showed that the ability of memory and leaning of rats that took regulating liver Qi and tonifying kidney formula performed better than model group, as the results of correct response rate, times of error reaction, standard required training times and total reaction time were all significant between LK group and model group (P<0.01, P<0.01, P<0.05 and P<0.05). The neurogranin mRNA level in CA1 region of rats hippocampus was also higher in LK group than that in model group (P<0.01). Conclusion: Memory and learning ability will be damaged by combined fatigue model. By regulating liver Qi and tonifying the kidney can restore the damage of memory and learning ability and affect the level of neurogranin mRNA in the CA1 region of rats hippocampus.

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