| 曹银利唐成和李树军常晓崔勤涛.脑源性神经营养因子基因多态性与注意缺陷多动障碍的关联性研究[J].现代生物医学进展英文版,2011,11(2):317-319. |
| 脑源性神经营养因子基因多态性与注意缺陷多动障碍的关联性研究 |
| Association between Gene Polymorphism of Brain-derived NeurotrophicFactor and Attention Deficit Hyperactivity Disorder |
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| DOI： |
| 中文关键词: 脑源性神经营养因子 注意缺陷多动障碍 关联 |
| 英文关键词: Brain-derived neurotrophic factor Attention deficit hyperactivity disorder Association |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨脑源性神经营养因子(Brain-derived neurotrophic factor, BDNF) G196A、C270T 及Val66Met 3 个单核苷酸多态性
（SNP）位点与注意缺陷多动障碍（ADHD）的关系。方法：选取无亲缘关系的ADHD患者共114例，健康对照共96例。采用聚合酶
链反应-限制性片段长度多态性（PCR-RFLP）技术检测G196A、C270T和Val66Met 3 个多态性位点的多态性，采用HaploView4.0
及SPSS13.0软件进行连锁不平衡分析并比较两组基因型分布和等位基因频率。结果：BDNF三个多态性位点基因型及等位基因
频率分布均符合Hardy-Weinberg 定律。ADHD 组G196A 和C270T 多态性位点分布与正常对照组比较差异无统计学意义，而
BDNF基因Val66Met位点的基因型及等位基因频率分布在ADHD组与对照组存在显著性差异(p<0.05)，ADHD 组Val66Met 位
点的等位基因G（Val）频率显著高于正常对照组。结论：BDNF基因Val66Met 多态性可能与ADHD 发病有关，携带有Val66Met
多态性位点G等位基因的个体可能更容易产生ADHD。 |
| 英文摘要: |
| Objective: To investigate the relationship between the G196A, C270T and Val66Met polymorphisms of Brain-derived
neurotrophic factor (BDNF) gene and attention deficit hyperactivity disorder(ADHD). Methods: Genomic DNA was isolated from the
venous blood leukocytes of 114 unrelated patients with ADHD based on DSM-IV criteria and 96 healthy unrelated individuals (control
group). Polymorphism of G196A, C270T and Val66Met, were genotyped by PCR-restriction fragment length polymorphisms
(PCR-RFLP). The software HaploView4.0 and SPSS13.0 was used to detect the linkage disequilibrium, Genotype and allele frequencies
of the SNPs of BDNF gene in the ADHD and control group. Results: The genotypic distributions of these 3 SNPs did not deviate significantly
from Hardy-Weinberg equilibrium (p>0.05). There were significant differences in both allele and genotype frequencies of
Val66Met between the ADHD and control group (p<0.05). The allele G of Val66Met was significantly higher than that of controls
(x2=9.714, p=0.002，OR=0.522, 95%CI=0.347～0.788). No significant differences in both allele and genotype frequencies of G196A and
C270T was found between the ADHD and control group (p>0.05). Conclusion: There was reltionship between Val66Met polymor- phism
and ADHD. The individuals with G (Val) allele of Val66Met were susceptible to ADHD. |
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