| 谢利芳焦凯△.胰岛素对βTC-3 细胞胰岛素受体表达的作用[J].现代生物医学进展英文版,2011,11(1):108-110. |
| 胰岛素对βTC-3 细胞胰岛素受体表达的作用 |
| Effect of Insulin on Expression of Insulin Receptor in TC-3 Cell |
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| DOI： |
| 中文关键词: 胰岛素 胰岛素受体 βTC-3 细胞 胰岛素抵抗 |
| 英文关键词: Insulin Insulin receptor βTC3 cells Insulin resistance |
| 基金项目: |
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| 中文摘要: |
| 目的：观察外源性胰岛素对小鼠胰岛β 细胞瘤细胞株βTC-3 细胞胰岛素受体表达的影响。方法：采用免疫荧光细胞化学技
术结合激光扫描共聚焦显微镜观察高浓度胰岛素（100 IU/ml）刺激不同时间（0 min、30 min、60 min、120 min、240 min），培养的
βTC-3 细胞胰岛素受体的表达，用Image pro plus 软件对胰岛素受体的荧光强度进行了半定量分析。结果：与0 min 比较，胰岛素
孵育30 min、60 min、120 min、240 min 时胰岛素受体荧光强度均明显下降（P<0.05）。结论：高浓度胰岛素孵育βTC3 细胞后，可明
显下调胰岛素受体的表达，这可能是高胰岛素血症导致胰岛素抵抗产生的机制之一。 |
| 英文摘要: |
| Objective: To explore the effects of exogenous insulin on the expression of insulin receptors in mouse insulinoma
TC-3 cells. Methods: The expression of insulin receptors in TC-3 cells in different incubation time (0 min, 30mins, 60mins, 120mins,
240mins) exposure to 100 IU/ml insulin was analyzed by immune fluorescence cytochemistry combined with laser scanning confocal
microscopy. Semi-quantitative analysis of fluorescence intensity of insulin receptors was determined by Image Pro Plus 6.0 software.
Results: The expression of insulin receptors at 30,60,120 and 240 min was decreased significantly compared with that of 0 min incubated
with 100 IU/ml insulin (P<0.05). Conclusion: The expression of insulin receptor in βTC3 cells was obviousiy decreased with high concen-
tration insulin incubation, which may be one of the mechanism of insulin resistance caused by hyperinsulinemia. |
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