| 方征宇△ 张巨峰刘立李佳娜黎满慧.组胺H4 受体在胃腺癌组织中的表达及临床意义[J].现代生物医学进展英文版,2011,11(1):81-83. |
| 组胺H4 受体在胃腺癌组织中的表达及临床意义 |
| Expression of Histamine Receptor H4 in Gastric Adenocarcinoma and ItsClinical Significance |
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| DOI： |
| 中文关键词: 胃腺癌 组胺H4 受体 荧光实时定量PCR 免疫组化 |
| 英文关键词: Gastric adenocarcinoma Histamine Receptor H4 Immunohistochemistry Real-time PCR |
| 基金项目:中国博士后基金项目(20080440779) |
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| 中文摘要: |
| 目的：研究胃癌腺癌(gastric adenocarcinoma, GAC)中组胺H4 受体的表达水平及其临床意义。方法：60 例GAC 组织（病例
组）与配对癌旁组织（adjacent normal tissue, ANT）中应用免疫组织化学技术检测组胺H4 受体的表达,应用实时荧光定量RT-PCR
方法检测组胺H4 受体mRNA 的表达，统计分析组胺H4 受体表达与临床病理特征之间的关系。结果：①胃腺癌组织中组胺H4
受体蛋白的阳性表达率（11.7%）显著低于癌旁正常组织（96.7%）。②胃腺癌组织中组胺H4 受体mRNA 水平较癌旁组织明显降低
（p<0.001）。③组胺H4 受体蛋白和mRNA 表达异常和肿瘤的病理分级有相关性（p=0.0027 和p=0.0011），也与有无胃周淋巴结转
移有关（p<0.001 和p=0.0049）。结论：组胺H4 受体在胃腺癌组织有表达异常，表达量与病理分期相关。组胺H4 受体表达异常和
组胺水平紊乱可能在胃癌发生发展过程中有重要作用。 |
| 英文摘要: |
| Abjective: To investigate the expression level of HRH4 in gastric adenocarcinoma (GAC) and its clinical significance.
Methods: The protein and mRNA level of HRH4 was examined in tissues from 60 patients with GAC by SP immunohistochemistry and
real-time PCR compared with adjacent normal tissues (ANT). Results:①The positive expression rate of HRH4 protein in GACs (11.7%)
was significantly lower than that in ANTs（96.7%，P<0.001）.②The average mRNA level in patients with was much lower than those in
adjacent tissues. ③The positive expression rate of HRH4 protein and HRH4 mRNA in stage T3～T4 (2.6% and 5.1%) was significantly
lower than that in stage T1～T2 (28.6％ and 38.1%, P<0.05). Conclusion: The expression of HRH4 gene is reduced significantly in GAC
tissues and is correlated with TNM stage. Abnormally expressed HRH4 and histamine might play a role in the progression of GAC. |
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