| 刘小红1 沈阳.艰难梭菌毒素A 对胆管癌细胞株FRH-0201 增殖和凋亡的影响[J].现代生物医学进展英文版,2011,11(1):60-62. |
| 艰难梭菌毒素A 对胆管癌细胞株FRH-0201 增殖和凋亡的影响 |
| Effect of Clostridium DifficileToxin A on Proliferation and Apoptosis ofFRH-0201 Cholangiocarcinoma Cell Line |
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| DOI： |
| 中文关键词: 胆管肿瘤 细胞凋亡 艰难梭菌毒素A |
| 英文关键词: Billary tract neoplasms apoptosis Clostridium difficile toxin A |
| 基金项目: |
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| 中文摘要: |
| 目的：研究艰难梭菌毒素A(TcdA)对人胆管癌细胞株FRH-0201 细胞凋亡影响及作用机制。方法：采用MTT 法检测细胞增
殖抑制率，荧光染色检测TcdA 作用后细胞形态学变化，流式细胞术检测细胞凋亡，免疫细胞化学法检测Bcl-2 表达水平，Caspase
3 活性检测试剂盒检测Caspase-3 的活性。结果：TcdA 能抑制胆管癌细胞株FRH-0201 细胞增殖且呈时间、剂量依赖性，荧光染色
和流式细胞仪检测到细胞凋亡，与对照组相比，TcdA 作用后Bcl-2 蛋白表达下降，差异有统计学意义(P<0.05),Caspase-3 活性增
强，差异有统计学意义(P<0.05)。结论：TcdA 可以通过下调Bcl-2 蛋白表达表达，激活Caspase-3 而诱导FRH-0201 细胞凋亡。 |
| 英文摘要: |
| Objective: To investigate the proliferation inhibition and apoptosis effect of Clostridium difficile toxin A on the
cholangiocarcinoma cell lines FRH-0201. Methods: The cytotoxic activity of TcdA on FRH-0201 cells was tested by the MTT assay,
Morphological changes were observed by fluorescence microscopy. Apoptosis was examined by flow-cytometric analysis. The expression
of Bcl-2 protein was examined by SABC immunocytochemistry staining, and Caspase-3 activity was determined with a microplate
reader. Results: TcdA inhibited the proliferation of FRH-0201 cell line in a time- and dose-dependent manner, the cells presented the typical
morphological changes of apoptosis, the expression of Bcl-2 protein was down-regulated compared with control group (P<0.05), in
addition,Caspase-3 was activated significantly compared with control group (P<0.05). Conclusions: TcdA can induce apoptosis in
FRH-0201 cells by the down-regulation of Bcl-2 protein and activity of Caspase-3. |
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