| 汪丹丹李呼伦王菁华刘怡希王广友孙博刘希君穆莉莉△.模拟微重力诱导PC12 细胞衰老的初步探讨[J].现代生物医学进展英文版,2011,11(1):52-55. |
| 模拟微重力诱导PC12 细胞衰老的初步探讨 |
| Influence of Simulated Microgravity on Cellular Senescenceof PC12 Cells |
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| DOI： |
| 中文关键词: 细胞衰老 衰老相关β半乳糖苷酶 模拟微重力 PC12 细胞 |
| 英文关键词: cell senescence SA-β-gal simulated microgravity PC12 cell |
| 基金项目:黑龙江医药卫生科技计划项目（2009-206），哈尔滨医科大学研究生创新基金（HCXB2008004） |
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| 中文摘要: |
| 目的：构建模拟微重力条件下PC12 细胞的培养体系，探讨模拟微重力对PC12 细胞衰老的影响。方法：用Cytodex-3 型微载
体作为PC12 细胞的贴附载体，旋转细胞培养系统所提供10-2g 的微重力环境进行模拟微重力条件下的细胞培养。在倒置显微镜
下观察PC12 细胞的生长情况；用扫描电镜观察PC12 细胞超微结构的变化；衰老相关β 半乳糖苷酶（SA-β-gal）特异性染色对
衰老的PC12 细胞进行评估。结果：光镜下模拟微重力培养的PC12 细胞表现出类衰老细胞的形态，扫描电子显微镜下观察发现其
微绒毛增多。SA-β-gal 染色的结果显示在模拟微重力的作用下，PC12 细胞SA-β-gal 的活性升高。结论：模拟微重力可以引起
PC12 细胞衰老样的形态变化，以及SA-β-gal 的活性升高。 |
| 英文摘要: |
| Objective: To develop PC12 cell culture system under the condition of simulated microgravity and to investigate the
influence of simulated microgravity on cellular senescence of PC12 cells. Methods: Cytodex-3 micro-carriers were used as the adherent
carrier of PC12 cells. The microgravity environment was simulated by a rotary cell culture system. The growth of PC12 cells cultured on
micro-carriers was detected by inverted microscope. The ultrastructure was detected by the scanning electron microscope. Senescence-associated
β-galactosidase (SA-β-gal) was employed to distinguish the senescent PC12 cells. Results: Simulated microgravity induced
senescent-liked morphologic changes, the microvillus were abundance on simulated microgravity cultured PC12 cells. The SA-β-gal
activity was upregulated by simulated microgravity culture. Conclusion: Simulated microgravity induced senescent-liked morphologic
changes and upregulated SA-β-gal activity. |
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