| 雷坚1 王慷慨2△ 刘小柳2 王桂良1 刘瑛2 刘梅冬2 肖献忠2.氯化钴对H9c2 心肌细胞新基因Mipu1 表达的影响[J].现代生物医学进展英文版,2011,11(1):30-0. |
| 氯化钴对H9c2 心肌细胞新基因Mipu1 表达的影响 |
| Effects of Cobalt Chloride on the Expression of a Novel Gene Mipu1in H9c2 Cardiomyoblast |
| |
| DOI： |
| 中文关键词: 氯化钴 Mipu1 H9c2 心肌保护 |
| 英文关键词: Cobalt chloride Mipu1 H9c2 myocardium protection |
| 基金项目:国家自然科学基金面上项目（30770855） |
|
| Hits: 747 |
| Download times: 1042 |
| 中文摘要: |
| 目的：研究氯化钴（CoCl2）对大鼠胚胎心脏来源的H9c2 心肌细胞中新基因Mipu1 表达的影响。方法：利用不同浓度的CoCl2
（0、100、200、300、400、500μmol/L）处理H9c2 细胞9h,及200μmol/L CoCl2 处理H9c2 细胞不同的时间(0、6、9、12、24h)后，用
RT-PCR 和Western Blot 分别观察H9c2 细胞Mipu1 mRNA 和蛋白的表达情况。结果：CoCl2 可以诱导H9c2 细胞中Mipu1 mRNA
和蛋白表达升高，200μM CoCl2 处理组的Mipu1 的表达水平高于100μM CoCl2 处理组，但是更高浓度的CoCl2(>200μM)不能
使Mipu1 的表达进一步升高。随着CoCl2 作用时间的延长，Mipu1 的表达逐步升高，在12h 达到高峰，但是在24h 后下降。结论：
CoCl2 能够促进H9c2 细胞新基因Mipu1 的表达，并且具有一定的剂量和时间依赖性。 |
| 英文摘要: |
| Objective: To investigate the effect of Cobalt chloride （CoCl2）on the expression of a novel gene Mipu1 in H9c2 rat
cardiomyocytes. Methods: Various concentration of CoCl2 （0,100,200,300,400,500μmol/L）was used to treat H9c2 cells for 9h, and
200μmol/L CoCl2 was used to treat H9c2 cells for various periods of time (0,6,9,12,24h), then RT-PCR and Western Blot were used to
observe the expression of Mipu1 in H9c2 cells. Results: The expression of Mipu1 mRNA and protein was enhanced by CoCl2 in H9c2
cells. Compared with the group of 100μmol/L CoCl2, the expression of Mipu1 was higher in the group of 200μmol/L CoCl2. However,
the expression of Mipu1 did not improve treated by more higher concentration of CoCl2(>200μM). With the time of CoCl2 treatment extending,
the expression of Mipu1 gradually increased, and reached peak at12h, but decreased at 24h. Conclusion: Treatment of H9c2 rat
cardiomyocytes with CoCl2 resulted in a limitedly dose-and time-dependent increase in a novel gene Mipu1 expression. |
|
View Full Text
View/Add Comment Download reader |
| Close |
|
|
|
