| 米 娜 刘全宏 张小翠.超声激活血卟啉对S180 肿瘤细胞表面EGFR 表达量的影响[J].现代生物医学进展英文版,2006,6(8):7-9. |
| 超声激活血卟啉对S180 肿瘤细胞表面EGFR 表达量的影响 |
| Effect of ultrasound activating hematophorphyrin on theexpression of EGFR in ascites S180 cell membrane |
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| DOI： |
| 中文关键词: 声动力疗法 血卟啉 超声 EGFR |
| 英文关键词: Sonodynamic therapy(SDT) Hematophorphyrin Ultrasound EGFR |
| 基金项目:国家自然科学基金资助项目(No .39870240 ;No .30270383) |
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| 中文摘要: |
| 目的:探讨超声激活血卟啉处理S180 肿瘤细胞后膜表面EGFR 表达量的变化。方法:将腹水瘤细胞随机分为四组, U 组和
UH 组细胞分别于频率1.8MHz、2 .0W/ cm2 的超声装置中照射3min, 并分别在1h 3h 5h 后取材, 应用免疫组化方法在光镜下观
察EGFR 的表达情况。结果:1h、3h 取材, U 组和UH 组平均光密度值明显低于CT 组和H 组, UH 组最低。而5h 取材时, UH 组平
均光密度值显著下降, 其它组基本无变化。结论:提示在高频低强度处理下, 随着时间的延长, 超声激活HpD 对EGFR 的抑制作
用增加, 显示可能是基因调控使EGFR表达下调, 从而使肿瘤细胞增殖减慢。 |
| 英文摘要: |
| Objective:To study the change of expression of EGFR in ascites S180 cell membrane during the process of ultrasound activating
hematophorphyrin.Methods:After being divided into four groups in random(U, UH, CT and H group), the tumor cells of U and UH groups
have been exposed to ultrasound at 1.80MHz and 2 .0W/ cm2 for 3min by using HpD.After 1h, 3h and 5h, the tumor cells in the four groups
were observed under optic microscope.Results:EGFR in CT an H groups has a higher expression in S180 ascites cells after 1h and 3h exposed
to ultrasound together with HpD.EGFR in UH group has a distinct decrease after 5h exposed to ultrasound together with HpD, while other three
groups have no changes.Conclusion:High frequency and low intensity ultrasound activating hematophorphyrin could inhibit EGFR, slow down
the multiplication of cancer cell , which suggests that gene may be involved in regulating the expression of EGFR in SDT. |
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