| 肖 辉 金德均 王 超 徐秀玉 李晓丹.survivin 基因在化疗药物诱导的
喉鳞癌Hep- 2 细胞凋亡中的作用[J].现代生物医学进展英文版,2006,6(6):13-15. |
| survivin 基因在化疗药物诱导的
喉鳞癌Hep- 2 细胞凋亡中的作用 |
| The role of survivin on the apoptosis of laryngeal squamouscell carcinoma Hep- 2 strain Induced by chemotherapeutical drugs |
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| DOI： |
| 中文关键词: survivin 基因 喉鳞癌Hep- 2 细胞 顺铂 三氧化二砷 |
| 英文关键词: Survivin gene Laryngeal squamous cell carcinoma Hep- 2 strain Cisplatin Arsenic trioxide |
| 基金项目:黑龙江省卫生厅科研课题资助, 编号2004- 133 |
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| 中文摘要: |
| 目的: 探讨survivin 基因在化疗药物三氧化二砷( As2O3) 和顺铂( DDP) 诱导的喉鳞癌(Hep- 2) 细胞凋亡中的作用。方
法: 用Hep- 2 细胞株进行培养传代, 以不同浓度的As2O3 和DDP 作用于Hep- 2 细胞, MTT 观察As2O3 和DPP 对Hep- 2 细胞生长
的抑制情况,TUNEL 法检测细胞凋亡数量, 用RT- PCR 方法检测survivin 基因的表达。结果: As2O3 和DDP 对Hep- 2 细胞的生长
均有抑制作用, 具有时间- 剂量依赖性, 化疗药物组细胞凋亡率和survivin 基因表达抑制率高于对照组。结论: 在As2O3 和DPP 诱
导的Hep- 2 细胞凋亡中survivin 基因表达被抑制, survivin 基因可能在DPP 和As2O3 抗肿瘤中发挥重要作用。 |
| 英文摘要: |
| Objective: To study the role of survivin gene on the apoptosis of the laryngeal squamous cell carcinoma Hep- 2 strain induced
by arsenic trioxide ( As2O3) and cisplatin ( DDP) . Methods: The laryngeal squamous cell carcinoma Hep- 2 strain was chosen in this
experiment. The inhibitory effects of chemotherapeutical drugs on Hep- 2 strain were assayed with MTT test. Apoptosis was detected by TUNEL
test. Expression of survivin gene was detected by reverse transcriptase PCR. Results: Both As2O3 and DDP obviously inhibited the laryngeal
squamous cell carcinoma Hep- 2 cell growth with dose and time- dependent. Apoptosis and inhibitory rate of survivin gene expression induced by
As2O3 and DDP was higher than that in control group. Conclusions: The survivin gene expression was inhibited during the course of apoptosis
in the laryngeal squamous cell carcinoma Hep- 2 strain induced by As2O3 and DDP. The survivin gene may play a critical role on the inhibiting
carcinoma of As2O3 and DDP. |
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