| 马 燕 牛小娟 李 静 高 霞 李兴玉.MNP- hTR 复合物对K562 细胞增殖影响的实验研究[J].现代生物医学进展英文版,2006,6(5):22-23. |
| MNP- hTR 复合物对K562 细胞增殖影响的实验研究 |
| The study on the effect of the MNP- hTR compound on theApoptosis and proliferation of K562 Cells |
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| DOI： |
| 中文关键词: MNP- 端粒酶反义核酸复合物 K562 细胞凋亡 荧光检测 集落形成 |
| 英文关键词: Antisense oligonucleotide with hTR K562 cells Fluorescence detection Cloning formation unit |
| 基金项目:上海市教育委员会高校发展基金( No. 05DC21) |
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| 中文摘要: |
| 探讨MNP- 端粒酶反义寡核苷酸复合物, 对K562 细胞凋亡和增殖的影响。应用荧光显微镜检测法和集落形成法, 分
别观察了凋亡细胞的形态学变化和细胞增殖能力。MNP- 端粒酶反义核酸复合物可诱导K562 细胞凋亡和抑制细胞增殖, 两实
验组数值经统计学处理, 具有非常显著性差异( p 分别< 0. 001) 。由此认为,MNP- 端粒酶反义核酸复合物对K562 白血病细胞具
有促进凋亡的作用, 此效果与剂量呈依赖性关系( r= 0. 992) 。 |
| 英文摘要: |
| To investigate the influence of the magnetic nanoparticles (MNP) - tolemorase antiense oligonucleotide compound to the
K562 cells, especially about the apoptosis and proliferation. Fluorescence microscope was used to watch the changing morphological characters of
K562 cells, and we also used the clonoly forming unit to detect the proliferation. The apoptosis and inhibit the proliferation of K562 cells might
was induced by the MNP- tolemorase antisense oligonucleotide compound. According to the statistical analysis of the experimental data, the difference
is significantly ( p< 0. 01- 0. 001) compared with the control groups, respectively. TheMNP- tolemorase antisense oligonucleotide compound
can promote cell apoptosis, besides, the effect depends on the dosage of the comound strictly( r= 0. 998) . |
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