| 王 青1、2 曾耀英1* 何贤辉1 肇静娴1 王 通1.SARS- CoV X4 基因转染对T 细胞
TNF- A及IL- 6 分泌的影响[J].现代生物医学进展英文版,2006,6(5):1-4. |
| SARS- CoV X4 基因转染对T 细胞
TNF- A及IL- 6 分泌的影响 |
| Study on cytokine secretion of T cell after SARS- CoV X4 gene transfection |
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| DOI： |
| 中文关键词: 严重急性呼吸综合症 冠状病毒 基因转染 细胞因子 T 淋巴细胞 |
| 英文关键词: Severe acute respiratory syndrome( SARS) Coronavirus Gene transfection Cytokine T- lymphocytes |
| 基金项目:国家重点基础研究发展规划项目( 973) 基金( NO. G1999054303) 和国家自然科学基金重点项目( NO. 30230350) 资助。 |
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| 中文摘要: |
| 目的: 通过检测SARS- CoV X4 基因转染后T 细胞细胞因子分泌的变化, 研究SARS- CoV X4 蛋白潜在的功能及其作
用机制, 并探讨其与SARS- CoV 致病机制的关系。方法: 利用RosetteSep 分离法分离外周血T 细胞, 以Amaxa 核转染仪将pEGFP
- SARS- CoV X4 真核表达载体转入T 细胞, 应用激光共聚焦显微镜观察其瞬时表达情况, 流式细胞仪检测转染效率, CBA 技术
检测T 细胞细胞因子。结果: SARS- CoV X4 基因转染后12h 得到了明显表达, 共聚焦显微镜观察到EGFP 绿色荧光表达, 流式细
胞仪检测其表达率为245; 与空载体转染组相比, SARS- CoV X4 基因转染能显著促进T 细胞IL- 6 及TNF- A的分泌。结论:
SARS- CoV X4 蛋白可诱导T 细胞分泌前炎症因子IL- 6 及TNF- A, SARS- CoV X4 蛋白可能在SARS 发病机制中起重要的作用。 |
| 英文摘要: |
| Objective: To explore the function of the SARS- CoV X4 protein and its role in SARS pathogenesis, by detecting the
change of cytokine secretion of T cells after transfection of SARS- CoV X4 gene. Methods: T cells were transfected with pEGFP- SARS- CoV
X4 expression vector by Amaxa Nucleofector , and the green fluorescence of EGFP were detected with flow cytometry and confocal microscopy;
The production of cytokine were investigated by Cytometry Beads Assay. Results: The green fluorescence of EGFP could be detected by with
flow cytometry, confocal microscopy; the secretion of IL- 6 and TNF- Aincreased remarkably in SARS- CoV X4 transfection group. Conclusion:
SARS- CoV X4 can promote T cells to secrete preinflammatory cytokine IL- 6 and TNF- A; it may play an important role in the pathogenesis
of SARS. |
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