| 朱传合 贺亚男 路福平 杜连祥.微波对阿维拉霉素产生菌诱变效应的研究[J].现代生物医学进展英文版,2006,6(4):32-34. |
| 微波对阿维拉霉素产生菌诱变效应的研究 |
| A Study on Mutagenic effects of Avilamycin-producing Strain Induced by Microwave Irradiation |
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| DOI： |
| 中文关键词: 微波 阿维拉霉素 诱变效应 |
| 英文关键词: Microwave Avilamicin Mutagenic effects |
| 基金项目: |
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| 中文摘要: |
| 阿维拉霉素产生菌SV 微波诱变效应的研究发现:菌株SV 对微波敏感(微波处理60s , SV 菌株存活率低于10 %), 菌落
形态变化大。微波诱变处理最佳作用方式为培养皿不加盖、快速冰上冷却, 最佳处理时间为50s(其正突变率25.3%)。通过微波
诱变处理、阿维拉霉素推理筛选, 最终获得一株稳定性良好, 阿维拉霉素产量达到21 .5mg/ L, 较出发菌株提高119.4 %的突变株
SV-15。 |
| 英文摘要: |
| To investigate Mutagenic effects of Avilamycin-producing Strain induced by microwave irradiation .The experimental results
indicated that the capacity of SV strain to microwave irradiation was sensitive (its survival rate was under 10% for 60s radiation).Not sealing
the culture dish and fast cooling it was the optimal irradiation mode of microwave , and 50 seconds was the optimal irradiation time of microwave
(positive mutation rate , 25.3%).To obtain high -yield avilamycin -producing strain, 50s microwave irradiation and screening of avilamycinrational
mutant is used for the study on screening mutation .SV-15 mutants were obtained , and the mutant could produce 21.5mg/ L avilamy cin ,
which was 2.194 times as much as the potency of original strain by flake fermentation. |
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