| 莫晓媚 李 静 李延团 耿美玉.一种钒配合物LMC 抑制DNA 拓扑异构酶?的抗肿瘤作用[J].现代生物医学进展英文版,2006,6(4):13-15. |
| 一种钒配合物LMC 抑制DNA 拓扑异构酶?的抗肿瘤作用 |
| The antitumor activity of a vanadium compound LMCin inhibiting DNA topoisomerase |
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| DOI： |
| 中文关键词: 钒配合物 DNA 拓扑异构酶 抗肿瘤 |
| 英文关键词: Vanadium compound DNA topoisomerase Antitumor |
| 基金项目:国家重点基础研究发展规划( 973 计划) 资助项目(No. 2003CB716400) |
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| 中文摘要: |
| 目的: 探讨钒配合物LMC 对拓扑异构酶? 、ò (Topo- ? 、Topo- ò ) 的影响及其抗肿瘤活性。方法: 采用DNA 松弛实
验观察LMC 对Topo- ? 、活性的影响并探讨其相关分子作用机制; 采用MTT 法、流式细胞术在细胞水平观察了LMC 的抗肿瘤作
用。结果: LMC 可明显抑制Topo- ? 活性, 对Topo- ò 无明显抑制作用, 对多种肿瘤细胞株A549、Hela、BEL- 7402 具有明显抑制
生长的作用, 且可将细胞阻断在G2/M 期, 而对正常细胞株L- 02 生长无明显影响。结论: 钒配合物LMC 具有抑制Topo- ? 活性
而发挥抗肿瘤的作用。 |
| 英文摘要: |
| In this paper, we studied the effects of vanadium compound LMC on DNA topoisomerase and its antitumor activity. DNA
relaxation assay was used to detect the activity of LMC on topoisomerase ? ( Topo- ? ) and topoisomerase ò ( Topo- ò ) and its correlated
molecular mechanism of action; MTT assay and Flow cytometry were used to detect LMC. s antitumor activity from cytology angle. LMC can inhibit
Topo- I activity obviously, but not, Topo- II LMC can also inhibit A549、Hela、BEL- 7402 cell lines growth evidently, and LMC can arrest the
cells at G2/M stage. However, it makes no difference to normal cell line L- 02, from which we conclude that Vanadium compound LMC has obvious
anticancer activity through inhibiting Topo- I activity. |
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