| 李艳彦 谢 鸣 陈 禹 王洪海.肝郁脾虚证模型大鼠下丘脑- 垂体- 肾上腺皮质的变化[J].现代生物医学进展英文版,2006,6(4):10-12. |
| 肝郁脾虚证模型大鼠下丘脑- 垂体- 肾上腺皮质的变化 |
| Study on Change of Hypothalamus- pituitary- adrenal Axis(HPAA)in TCM?? s Syndrome Model of GYPX |
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| DOI： |
| 中文关键词: 肝郁脾虚证 动物模型 大鼠 HPA 轴 复合造模法 |
| 英文关键词: Syndrome of liver- qi stagnation and spleen- qi deficiency Animal model Rats HPAA A compound method of establishing
model |
| 基金项目:本课题为国家211 项目基金资助课题 |
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| 中文摘要: |
| 目的: 探讨复合造模法复制肝郁脾虚证大鼠模型的HPA 轴变化及特点。方法: 大鼠采用慢性束缚+ 过度疲劳+ 饮食
失节法, 连续三周, 自然恢复一周。观察造模期间各不同时间点大鼠肾上腺重量及指数、下丘脑促肾上腺皮质激素释放激素
( CRH) 、血浆促肾上腺皮质激素( ACTH) 及皮质酮( CORT) 的变化。结果: 与正常组比较, 模型大鼠肾上腺重量第一周显著增加( P
< 0. 001) , 第三、四周显著减低( P< 0. 001 和P< 0. 01) , 其肾上腺指数在实验各周均显著高于正常组( P< 0. 05 和P< 0. 01) ; 下丘脑
CRH 第三周显著低于正常组( P< 0. 05) , 第四周回升并显著高于正常组( P< 0. 05) ; 血浆ACTH 在第一、三、四周均显著降低( P< 0.
05) ; 血浆CORT 第一周显著降低( P< 0. 05) , 第二周有所回复, 第三、四周仍显著降低( P< 0. 05 和P< 0. 001) 。结论: 该肝郁脾虚证
大鼠模型存在HPA 轴的功能减低, 垂体对上位激素调节的反应能力减低可能是该模型的一个重要的生物学特征。 |
| 英文摘要: |
| Objective: To study the change of Hypothalamus- pituitary- adrenal axis( HPAA) in the TCM?? s syndrome model of
GYPX( stagnation of liver- qi and deficiency of spleen- qi) made by a new compound method. Methods: Chronic astricting、excess fatigue and
out of constant diet were adopted towards the rats for three weeks running and then the rats were restored naturally for one week. The changes of
the weight and index of rats?? adrenal gland and the levels of Corticotropin- releasing Hormone( CRH) in hypothalamus, AdrenocorticotropicHormone(
ACTH) and Corticotrophin( CORT) in plasmawere observed at different time during making model. Results: Compared with relevant normal
group, the weight of the model rats?? adrenal gland increased significantly at the 1st week( P< 0. 001) and decreased at the 3rd and 4th week
( P< 0. 001 and P< 0. 01) , but the index of adrenal gland increased significantly during the 4 weeks( P< 0. 05 and P< 0. 01) ; CRH in hypothalamus
decreased significantly at the 3rd week( P< 0. 05) , and brought back and increased significantly at 4th week( P< 0. 05) ; ACTH in plasma
decreased significantly at the 1st, 3rd and 4th week( P< 0. 05) ; CORT in plasma decreased significant in the 1st week( P< 0. 05) , went back a
little at 2nd week and decreased again at 3rd and 4th week( P< 0. 05 and P< 0. 001) . Conclusion: The functions of HPAA of the rats?? in
GYPX model were reduced. The lower reactive ability of pituitary to the adjustment of superior hormone may be the important biologic character of
this model. |
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