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目的: 探讨使用MEK- 6318K 血细胞分析仪时升高的胆红素对黄疸型肝病患者的白细胞结果影响程度及检测失败的原因, 并提出解决方法。方法: 对60 例黄疸型肝病患者的标本分成两组: 总胆红素在20- 100umol/ L 范围30 例, 在100. 1- 250umol/ L 范围30 例。均使用EDTA- K2 抗凝真空采集病人静脉血2ml, 分别用MEK- 6318K 血细胞分析仪和手工操作方法对同一标本进行血液细胞的分析测定。结果: 总胆红素在20- 100 umol/ L 范围内30 例患者的标本, 两种方法测定白细胞结果经统计学处理P> 0. 05 无显著性差别; 总胆红素在100. 1- 250umol/ L 范围内30 例患者的标本, 两种方法测定白细胞结果, 经统计学处理 P< 0. 001 有极显著性差别。结论: 在使用MEK- 6318K 血细胞分析仪检测高黄疸标本时一定要注意白细胞结果, 对报警标本, 可以采用静脉血10ul 测定模式, 但最好采用手工操作来检测标本, 这样才能为临床提供更准确结果。

英文摘要:

Objective: To investigate using MEK- 6318K hematology analyzer( HA) to detect the effect of elevated bilirubin{ BIL} on the white blood cell(WBC) count in the serum of icterohepatopath, and to explore the reason of failing and the method of solving . Methods: The specimens of 60 icterohepatopaths were divided into two groups: in the first group( n= 30) , total bilirubin( TBR) was 20~ 100 umol/ L; in the second group( n= 30) , TBR 100. 1~ 250umol/ L. Using EDTA- K2 anticoagulation vacuum collecting tube to collect each patient. s venous blood 2ml, the same specimen was detected by MEK- 6318K HA and manual operation, respectively. Results: In the first group, there was no signif-i cant difference in WBC count detected by the two kinds of methods( P> 0. 05) ; while in the second group, significant difference( P< 0. 001) . Conclusion: When using MEK- 6318K HA to detect the specimens of elevated BIL, WBC counts should be paid more attention to. For any alarm specimens, the determination mode of venous blood 10 ul can be adopted, but detecting by manual operation is best,which can offer more accurate data for the clinical application.

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