| 刘芙君1, 2 李建远1 王培林2 张成林1 王海燕1 王修海2.大鼠附睾蛋白质组的提取和二维液相色谱分离[J].现代生物医学进展英文版,2006,6(2):22-23. |
| 大鼠附睾蛋白质组的提取和二维液相色谱分离 |
| Extraction and two- dimensional liquid chromatographic fractionation of rat epididymal fluid proteins |
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| DOI： |
| 中文关键词: 蛋白质组 二维液相色谱分离 附睾蛋白质 |
| 英文关键词: Proteome Two dimensional liquid chromatographic fractionation Epididymal proteins |
| 基金项目: |
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| 中文摘要: |
| ?? 背景与目的: 提取大鼠附睾液蛋白并建立一种利用二维液相色谱法分离附睾蛋白组的方法。方法: 分离提取大鼠附
睾液蛋白, 样品利用起始缓冲液置换后, 进行一维色谱聚焦分离, 然后收集pH8. 5- 4. 0 之间的组份进行二维反相高压液相色谱
分离, 最后将获得的二维UV 图通过ProteoVue 软件转换成PI/ UV 图谱。结果: 成功提取了附睾液蛋白, 并通过二维液相色谱成功
建立了大鼠头体尾部附睾液蛋白的二维PI/ UV 图谱, 收集了一维色谱聚焦分离的pH8. 5- 4. 0 区间的20 个组份, 并将每个组份
进行二维色谱分离后转换为PI/ UV 图谱。结论: 为进一步全面研究附睾蛋白功能和体液差异蛋白质组研究打下了基础。 |
| 英文摘要: |
| Background and Objective: To extract rat epididymal fluid proteins and develop an approach for fractionating the proteins by
two dimensional liquid chromatography. Methods: The epididymal fluid proteins extracted from three rats were exchanged with start buffer and
separated by chromatofocusing in the first dimension, the fractions between pH8. 5 and pH4. 0 collected from 1D were separated by non- porous
silica reverse- phase HPLC. The UV maps were transformed into PI/ UV maps by ProteoVue software. Results: We successfully extracted ep-i
didymal fluid proteins and 20 fractions between pH8. 5- pH4. 0 were collected, each fraction was separated by 2D and the UV maps were transformed
into PI/ UV maps. Conclusion: Our study laid a foundation for further studies of epididymal fluid proteins and other body fluid Differential
Proteome. |
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