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| 盆腔炎性疾病后遗症大鼠模型优化及机制探讨* |
| Optimization study of rat models for sequelae of pelvic inflammatory disease* |
| 投稿时间：2025-02-21 修订日期：2025-02-28 |
| DOI： |
| 中文关键词: 盆腔炎性疾病后遗症 SPID 动物模型 细菌感染 |
| 英文关键词: Sequelae of pelvic inflammatory disease, SPID, Rat models, Bacterial infections. |
| 基金项目:新教师启动基金项目 |
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| 摘要点击次数: 18 |
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| 中文摘要: |
| 探索建立符合临床特征的、稳定的盆腔炎性疾病后遗症（sequelae of pelvic inflammatory disease，SPID）大鼠疾病模型，为研究SPID的发病机制和治疗提供可靠的实验模型。方法：采用机械损伤联合菌液注射方法制备SPID模型。将24只7周龄SD大鼠分为假手术组、模型组-A（108 cfu/mL混合菌液，0.2 mL）、模型组-B（109 cfu/mL混合菌液，0.2 mL）、模型组-C（108 cfu/mL大肠杆菌，0.2 mL）。称取大鼠子宫重量、计算子宫系数；采用全自动血细胞分析仪检测血常规；采用苏木精-伊红染色（HE）、马松染色（Masson）检测检测大鼠子宫组织病变；采用酶联免疫吸附法（ELISA）检测大鼠子宫组织匀浆中白介素-1β（IL-1β）、白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）；采用免疫蛋白印记法（Western blot）检测NF-κB信号通路相关蛋白表达情况。结果：与假手术组相比，3个模型组大鼠子宫系数显著上升（P<0.05，P<0.01）；模型组-A的白细胞（white blood cell，WBC）和中性粒细胞（neutrophil，NE）水平显著上升（P<0.01）；模型组-B的LY水平显著下降（P<0.01）；3个模型组的IL-1β、TNF-α水平显著升高（P<0.01），模型组-A、模型组-B的IL-6水平显著升高（P<0.05，P<0.01）；模型组-A、模型组-B胶原容积分数显著升高（P<0.01）。机制探索发现，与假手术组相比，模型组-A的p-IKKβ/IKKβ、p-IκBα/IκBα、p-p65/p65表达水平显著上升（P<0.01），IκBα/β-actin表达水平显著下降（P<0.01）；模型组-B的p-IKKβ/IKKβ表达水平显著上升（P<0.01）。结论：0.2 mL，108 cfu/mL混合菌液联合机械损伤能够成功构建符合临床特征的、稳定的SPID大鼠模型，该造模方法干预了NF-κB炎症信号通路的表达。 |
| 英文摘要: |
| To establish a stable rat model of sequelae of pelvic inflammatory disease (SPID) with clinical characteristics, and to provide a reliable experimental model for the study of the pathogenesis and treatment of SPID. Methods: Twenty-four 7-week-old SD rats were divided into sham operation group, model-A (108 cfu/mL mixed bacterial solution, 0.2 mL), model-B (109 cfu/mL mixed bacterial solution 0.2 mL), and model-C (108 cfu/mL E. coli 0.2 mL). A syringe needle was used to make 3 strokes back and forth on the endometrium to cause mechanical damage, and then 0.2 mL of bacterial solution was injected into the rat in utero. The weight of the rat's uterine was weighed and the uterine index was calculated. The automatic hematology analyzer was used to detect the blood routine; hematoxylin-eosin staining (HE) and masson staining were used to detect uterine tissue lesions in rats. Enzyme-linked immunosorbent assay (ELISA) was used to detect interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in rat uterine tissue homogenates. Western blot was used to detect the expression of proteins related to NF-κB signaling pathway. Results: Compared with the sham operation group, the uterine index of model-A, model-B, and model-C were significantly increased (P<0.05, P<0.01). The levels of WBC and NE in the model-A increased significantly (P<0.01). The LY level of model-B decreased significantly (P<0.01).The levels of IL-1β、TNF-α in model-A, model-B, and model-C were significantly increased (P<0.01). The levels of IL-6 in model-A and model-B were significantly increased (P<0.05, P<0.01). The collagen volume fractions of model-A and model-B were significantly increased (P<0.01). The expression levels of p-IKKβ/IKKβ、p-IκBα/IκBα and p-p65/p65 in model -A were significantly increased (P<0.01), and the expression levels of IκBα/β-actin were significantly decreased (P<0.01). The expression level of p-IKKβ/IKKβ in model-B was significantly increased (P<0.01). Conclusions: A stable rat model of SPID that conforms to clinical characteristics can be successfully constructed by combining 0.2 mL of mixed bacterial solution with a concentration of 10? cfu/mL and mechanical injury. This modeling method intervened in the expression of the NF-κB inflammatory signaling pathway. |
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