| 雷坳錡,陈姝谕,周雨欣,刘一妍,颜学兵.慢性乙型肝炎患者外周血SIRT1、SIRT3与HBV-DNA载量和氧化应激的相关性分析[J].,2025,(1):80-86 |
| 慢性乙型肝炎患者外周血SIRT1、SIRT3与HBV-DNA载量和氧化应激的相关性分析 |
| Correlation Analysis of SIRT1 and SIRT3 in Peripheral Blood with HBV-DNA Load and Oxidative Stress in Patients with Chronic Hepatitis B |
| 投稿时间：2024-07-29 |
| DOI：10.13241/j.cnki.pmb.2025.01.011 |
| 中文关键词: 慢性乙型肝炎 沉默信息调节因子1 沉默信息调节因子3 乙肝病毒脱氧核糖核酸载量 氧化应激 |
| 英文关键词: Chronic hepatitis B Silent information regulator 1 Silent information regulator 3 Hepatitis B virus deoxyribonucleic acid load Oxidative stress |
| 基金项目:江苏省自然科学基金项目（20KJB320012） |
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| 中文摘要: |
| 摘要 目的：分析慢性乙型肝炎（CHB）患者外周血沉默信息调节因子（SIRT）1、SIRT3与乙肝病毒脱氧核糖核酸（HBV-DNA）载量、氧化应激的相关性。方法：选择2021年2月至2024年1月于本院就诊的190例CHB患者，采用实时荧光定量聚合酶链反应技术法（RQ-PCR）测定HBV DNA载量及SIRT1、SIRT3 mRNA表达，酶联免疫吸附法测定氧化应激指标[丙二醛（MDA）、谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、NADPH氧化酶2（NOX2）]。按照HBV-DNA载量将CHB患者分为低载量组（n=64）、中载量组（n=81）、高载量组（n=45），对比3组上述指标，采用Spearman或Pearson相关性分析外周血SIRT1mRNA、SIRT3mRNA与HBV-DNA载量、氧化应激的关系。结果：高载量组SIRT1 mRNA表达最高，其次为中载量组、低载量组，高载量组SIRT3 mRNA表达最低，其次为中载量组、低载量组（P＜0.05）；高载量组MDA、NOX2最高，其次为中载量组、低载量组，高载量组GSH、SOD最低，其次为中载量组、低载量组（P＜0.05）；Spearman或Pearson相关性分析发现，HBV-DNA载量、MDA、NOX2与SIRT1 mRNA表达呈正相关（P＜0.05），与SIRT3 mRNA表达呈负相关（P＜0.05）；GSH、SOD与SIRT1 mRNA表达呈负相关（P＜0.05），与SIRT3 mRNA表达呈正相关（P＜0.05）。结论：随着HBV-DNA载量增加，CHB患者SIRT1 mRNA表达呈升高趋势、SIRT3呈降低趋势，且SIRT1、SIRT3mRNA表达与氧化应激密切相关。 |
| 英文摘要: |
| ABSTRACT Objective: To analyze the correlation between silent information regulator (SIRT) 1 and SIRT3 in peripheral blood of patients with chronic hepatitis B (CHB) and hepatitis B virus deoxyribonucleic acid (HBV-DNA) load and oxidative stress. Methods: A total of 190 patients with CHB who were treated in our hospital from February 2021 to January 2024 were selected. The HBV DNA load and the expression of SIRT1 and SIRT3 mRNA were determined by real-time fluorescence quantitative polymerase chain reaction (RQ-PCR). The oxidative stress indexes [malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), NADPH oxidase 2 (NOX2)] were determined by enzyme-linked immunosorbent assay. According to HBV-DNA load, CHB patients were divided into low load group (n=64), medium load group (n=81) and high load group (n=45). Compare the above three groups. Spearman or Pearson correlation was used to analyze the relationship between peripheral blood SIRT1 mRNA, SIRT3 mRNA and HBV-DNA load, oxidative stress. Results: The expression of SIRT1 mRNA in the high load group was the highest, followed by the medium load group and the low load group, and the expression of SIRT3 mRNA in the high load group was the lowest, followed by the medium load group and the low load group (P<0.05). MDA and NOX2 in the high load group were the highest, followed by the medium load group and the low load group. GSH and SOD in the high load group were the lowest, followed by the medium load group and the low load group (P<0.05). Spearman or Pearson correlation analysis showed that HBV-DNA load, MDA and NOX2 were positively correlated with SIRT1 mRNA expression (P<0.05), and negatively correlated with SIRT3 mRNA expression (P<0.05). GSH and SOD were negatively correlated with SIRT1 mRNA expression (P<0.05), and positively correlated with SIRT3 mRNA expression (P<0.05). Conclusion: With the increase of HBV-DNA load, the expression of SIRT1 mRNA in CHB patients showed an increasing trend and SIRT3 showed a decreasing trend, and the expression of SIRT1 and SIRT3 mRNA are closely related to oxidative stress. |
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