| 王 琼,宋忠亚,周雨潇,李佳音,鲍 丹.大肠埃希菌导致免疫缺陷小鼠死亡原因的初步研究[J].,2025,(1):11-18 |
| 大肠埃希菌导致免疫缺陷小鼠死亡原因的初步研究 |
| A Preliminary Study on the Cause of Death in Immunodeficient Mice Caused by Escherichia coli |
| 投稿时间：2024-07-02 |
| DOI：10.13241/j.cnki.pmb.2025.01.002 |
| 中文关键词: 大肠埃希菌 免疫缺陷 小鼠 致病菌 胃肠功能紊乱 |
| 英文关键词: Escherichia coli Immune deficiency Mice Pathogenic bacteria Gastrointestinal dysfunction |
| 基金项目:国家自然科学基金项目（82103765） |
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| 中文摘要: |
| 摘要 目的：探究实验室饲养的免疫缺陷SPF雌性小鼠在哺乳期异常死亡的原因。方法：对死亡的3只雌鼠、1只幼崽及1只对照哨兵鼠进行了解剖，取肝脏、脾脏、肠道及肛门组织进行了细菌培养及鉴定；将死亡小鼠及同笼架哨兵鼠的心脏、肺部、肝脏、脾脏、胃部及肠道组织进行病理切片及HE染色。结果：解剖发现雌鼠胃内大量的饲料积累，肠道基本为排空状态，内无粪便，肝脏、脾脏及肠道无异常表现。死亡雌鼠的心脏、肺部、肝脏及脾脏都有炎症细胞浸润；细菌培养结果显示异常死亡小鼠肝脏、脾脏、肠道及肛门处有细菌生长，对照哨兵鼠肝脏及脾脏无细菌生长。经时间飞行质谱仪鉴定异常死亡小鼠肝脏、脾脏、肠道及肛门处主要致病菌为大肠埃希菌，哨兵鼠肠道主要细菌为缓慢葡萄球菌及加氏乳杆菌，肛门处为土壤短芽孢杆菌。结论：本案例揭示了小鼠因胃肠功能紊乱而引发的内源性大肠埃希菌感染，这可能是导致免疫缺陷小鼠死亡的根本原因，提示在免疫缺陷小鼠饲养过程中必须严密监控粪便状况，以便及时发现并预防潜在的感染风险。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the causes of aberrant death in immunodeficient SPF female mice during lactation. Methods: Three female mice and their pups, as well as a control sentry mouse, were thoroughly dissected to determine the cause of death. The liver, spleen, intestine, and anus were cultured and identified. The heart, lung, liver, spleen, stomach, and intestinal tissues of both dead and sentry mice in the same cage were pathologically cut and stained with HE. Results: The stomach had accumulated a large amount of feed, the intestines were empty, and the liver, spleen, and intestines were free of stool. The heart, lungs, liver and spleen of the dead female mice were infiltrated with inflammatory cells. The bacterial culture indicated that the abnormal dead mice had bacterial growth in the liver, spleen, intestine, and anus. In contrast, the control mice had no bacterial growth in the liver and spleen. Escherichia coli was identified as the main pathogenic bacteria in liver, spleen, intestine and anus of abnormal dead mice by time-flight mass spectrometer. The main bacteria in the intestine of sentinel mice were staphylococcus lentus and Lactobacillus gasseri, and the main bacteria in the anus were Brevibacillus agri strain. Conclusion: This case reveals endogenous Escherichia coli infection caused by gastrointestinal dysfunction in mice, which may be the root cause of death in immunodeficient mice. This suggests that the fecal condition must be closely monitored during the feeding of immunodeficient mice to detect and prevent potential infection risks in time. |
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