| 张鸿宇,门伟业,熊紫怡,宋文君,党 雪,英振昊.基于线粒体相关的活性氧和谷胱甘肽探讨脑卒中的发病机制[J].,2024,(23):4595-4600 |
| 基于线粒体相关的活性氧和谷胱甘肽探讨脑卒中的发病机制 |
| Explore the Pathogenesis of Stroke Based on Mitochondrial Related Reactive Oxygen Species and Glutathione |
| 投稿时间：2024-05-16 修订日期：2024-06-18 |
| DOI：10.13241/j.cnki.pmb.2024.23.055 |
| 中文关键词: 线粒体 活性氧 谷胱甘肽 脑卒中 生物信息学 免疫浸润 |
| 英文关键词: Mitochondria Reactive oxygen species Glutathione Stroke Bioinformatics Immune infiltration |
| 基金项目:山东省中医药科技项目（2020M003） |
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| 摘要点击次数: 18 |
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| 中文摘要: |
| 摘要 目的：基于生物信息学探究线粒体相关的活性氧（ROS）和谷胱甘肽（GSH）的相关基因在脑卒中中的作用及临床意义，进行关键基因的筛选。方法：利用高通量基因表达数据库（GEO）获取脑卒中相关靶点，并通过FerrDb数据库获取线粒体相关的ROS和GSH的有关靶点。通过使用R语言，对线粒体相关的ROS和GSH的相关基因在脑卒中中的基因集打分与表达差异进行评估，并免疫浸润分析、聚类分析及聚类后亚组相关特征差异分析。结果：线粒体相关的ROS和GSH基因集在正常组与脑卒中组中有表达差异，且两组免疫细胞与炎症因子表达水平具有较大不同。聚类分析分出的亚组1与亚组2在临床表型特征、基因表达水平、免疫细胞比例、炎症因子水平及蛋白富集等方面均具有一定不同。结论：线粒体相关的ROS和GSH相关基因在脑卒中的免疫中发挥重要作用，且其关键基因的表达水平不同或可影响不同的免疫途径与临床表型特征。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the role and clinical significance of mitochondria related reactive oxygen species(ROS) and glutathione(GSH) related genes in stroke based on bioinformatics, and to screen the key genes. Methods: Stroke related targets were obtained by gene expression omnibus (GEO) database, and mitochondria related ROS and GSH were obtained by FerrDb database. R language was used to evaluate the gene set scores and expression differences of mitochondria associated ROS and GSH related genes in stroke, as well as immune infiltration analysis, cluster analysis and post-cluster subgroup related feature differences. Results: The expression of mitochondrial related ROS and GSH gene sets was different between the normal group and the stroke group, and the expression levels of immune cells and inflammatory factors were significantly different between the two groups. Subgroup 1 and subgroup 2 were different in clinical phenotype, gene expression level, proportion of immune cells, level of inflammatory factors and protein enrichment. Conclusion: Mitochondria-related ROS and GSH genes play an important role in the immunity of stroke, and the different expression levels of their key genes may affect different immune pathways and clinical phenotypes. |
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