| 时玲玲,吴桂杰,王 艳,李彦玲,刘春双.高雌激素暴露对妊娠期高血压孕鼠胎盘损伤
和免疫功能的调节作用研究[J].,2024,(23):4417-4420 |
| 高雌激素暴露对妊娠期高血压孕鼠胎盘损伤
和免疫功能的调节作用研究 |
| Study on the Regulatory Effect of High Estrogen Exposureon Placental Damage and Immune Functionin Pregnant Rats with Gestational Hypertension |
| 投稿时间：2024-07-02 修订日期：2024-07-30 |
| DOI：10.13241/j.cnki.pmb.2024.23.004 |
| 中文关键词: 高雌激素 妊娠期高血压 孕鼠 胎盘损伤 免疫功能 |
| 英文关键词: High estrogen Gestational hypertension Pregnant rats Placental damage Immune function |
| 基金项目:2022年度河北省医学科学研究课题计划项目（20220316） |
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| 中文摘要: |
| 摘要 目的：探讨高雌激素暴露对妊娠期高血压（GH）孕鼠胎盘损伤和免疫功能的调节作用。方法：构建GH大鼠模型，将大鼠随机分为对照组（Control组）、GH组、雌激素低剂量组（L-estrogen组）、雌激素中剂量组（M-estrogen组）、雌激素高剂量组（H-estrogen组）。检测孕鼠收缩压、舒张压、24 h尿蛋白、促卵泡激素（FSH）、雌二醇（E2）、促黄体生成素（LH）、肿瘤坏死因子-α（TNF-α）、γ干扰素（IFN-γ）、白细胞介素（IL）-6、IL-17含量、辅助性T细胞（Th）17/调节性T细胞（Treg）细胞比例；观察孕鼠胎盘组织病理变化和胎盘滋养细胞超微结构。结果：GH组孕鼠绒毛数量减少，滋养层细胞核固缩，收缩压、舒张压、24 h尿蛋白、Th17细胞比例、Th17/Treg比值、IFN-γ、TNF-α、IL-6、IL-17含量显著增加，FSH、E2、LH、Treg细胞比例显著降低（P<0.05）；与GH组比较，L-estrogen组、M-estrogen组、H-estrogen组孕鼠胎盘组织损伤、滋养细胞核固缩减轻，收缩压、舒张压、24 h尿蛋白、Th17细胞比例、Th17/Treg比值、IFN-γ、TNF-α、IL-6、IL-17含量显著降低，FSH、E2、LH、Treg细胞比例显著增加，且呈剂量依赖性（P<0.05）。结论：雌激素暴露可降低GH大鼠血压，改善GH孕鼠胎盘损伤和免疫功能，以高剂量雌激素的干预效果更佳。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the regulatory effect of high estrogen exposure on placental damage and immune function in pregnant rats with gestational hypertension (GH). Methods: GH rat model was constructed, and rats were randomly divided into control group (Control group), GH group, low-dose estrogen group (L-estrogen group), medium-dose estrogen group (M-estrogen group) and high-dose estrogen group (H-estrogen group). The systolic pressure, diastolic pressure, 24 h urine protein, follicle stimulating hormone (FSH), estradiol (E2) and luteinizing hormone (LH), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin (IL) -6, IL-17 contents, and the proportion of helper T cells (Th) 17/regulatory T cells (Treg) of pregnant rats were detected. The pathological changes of placental tissue and the ultrastructure of placental trophoblast cells in pregnant rats were observed. Results: The number of villi in GH group was decreased, the nucleus of trophoblast cells was pyknosis, systolic blood pressure, diastolic blood pressure, 24 h urine protein, Th17 cell ratio, Th17/Treg ratio, IFN-γ, TNF-α, IL-6 and IL-17 were significantly increased, and the proportion of FSH, E2, LH and Treg cells was significantly decreased (P<0.05). Compared with GH group, the placental tissue damage and nuclear pyknosis of trophoblast cells were reduced in L-estrogen group, M-estrogen group and H-estrogen group, systolic blood pressure, diastolic blood pressure, 24 h urine protein, Th17 cell ratio, Th17/Treg ratio, IFN-γ, TNF-α, IL-6 and IL-17 levels were significantly decreased, and the proportion of FSH, E2, LH and Treg cells was significantly increased in a dose-dependent manner (P<0.05). Conclusion: Estrogen exposure can reduce the blood pressure of GH rats, improve placental damage and immune function in GH pregnant rats, and the intervention effect of high-dose estrogen is better. |
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