| 张珍,刘光辉,梁慧玲,吴开松,张珒珒,董 翔.血清lncRNA NNT-AS1、miR-582-5p与支气管哮喘患儿气道炎症、
气道高反应的关系研究[J].,2024,(22):4256-4257 |
| 血清lncRNA NNT-AS1、miR-582-5p与支气管哮喘患儿气道炎症、
气道高反应的关系研究 |
| Research on the Relationship between Serum lncRNA NNT-AS1 and miR-582-5p and Airway Inflammation and Airway Hyperresponsivenessin Children with Bronchial Asthma |
| 投稿时间：2024-04-12 修订日期：2024-05-06 |
| DOI：10.13241/j.cnki.pmb.2024.22.015 |
| 中文关键词: lncRNA NNT-AS1 miR-582-5p 支气管哮喘 儿童 气道炎症 气道高反应 |
| 英文关键词: lncRNA NNT-AS1 miR-582-5p Bronchial asthma Children Airway inflammation Airway hyperresponsiveness |
| 基金项目:湖北省自然科学基金面上项目（2021CFB308） |
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| 中文摘要: |
| 摘要 目的：探讨血清长链非编码核糖核酸（lncRNA）烟酰胺核苷酸反义转氢酶RNA1（NNT-AS1）、微小核糖核酸（miR）-582-5p与支气管哮喘患儿气道炎症、气道高反应的关系。方法：选择120例支气管哮喘患儿，根据相关指南分为缓解期组（n=52）与发作期组（n=68）。另选择同期60例健康儿童作为对照组。对比三组血清lncRNA NNT-AS1、miR-582-5p相对表达量及气道炎症细胞因子[白介素-17（IL-17）、肿瘤坏死因子-α（TNF-α）]水平、气道反应性指标[基础呼吸阻力（Rrs cont）、最小诱发累积剂量（Dmin）、基础呼吸传导率（Grs cont）]。Pearson相关性检验分析血清lncRNA NNT-AS1、miR-582-5p表达与血清气道炎症细胞因子、气道反应性指标的关系。结果：缓解期组、发作期组lncRNA NNT-AS1相对表达量、血清IL-17、TNF-α水平、Rrs cont高于对照组，且发作期组高于缓解期组（P＜0.05）。缓解期组、发作期组miR-582-5p相对表达量、Grs cont、Dmin低于对照组，且发作期组低于缓解期组（P＜0.05）。支气管哮喘患儿血清lncRNA NNT-AS1表达与IL-7、TNF-α、Rrs cont呈正相关（r＞0，P＜0.05），与Grs cont、Dmin呈负相关（r＜0，P＜0.05）；血清miR-582-5p表达与IL-17、TNF-α、Rrs cont呈负相关（r＜0，P＜0.05），与Grs cont、Dmin呈正相关（r＞0，P＜0.05）。结论：支气管哮喘患儿血清lncRNA NNT-AS1呈高表达，miR-582-5p呈低表达，二者表达与患儿哮喘分期、气道炎症、气道高反应密切相关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between serum long non-coding RNA (lncRNA) nicotinamide nucleotide antisense transferase RNA1 (NNT-AS1), microRNA (miR)-582-5p and airway inflammation and airway hyperresponsiveness in children with bronchial asthma. Methods: 120 children with bronchial asthma were divided into remission group (n=52) and attack group (n=68) according to the relevant guidelines. Another 60 healthy children in the same period were selected as the control group. The relative expression of serum lncRNA NNT-AS1, miR-582-5p and serum airway inflammatory cytokines [interleukin-17 (IL-17), tumor necrosis factor-α (TNF-α)], airway responsiveness indicators [basic respiratory resistance (Rrs cont), minimum induced cumulative dose (Dmin), basic respiratory conductivity (Grs cont)] were compared among three groups. The relationship between serum lncRNA NNT-AS1, miR-582-5p expression and airway inflammatory cytokines, airway responsiveness indicators were analyzed by Pearson correlation test. Results: The relative expression of lncRNA NNT-AS1, serum levels of IL-17, TNF-α and Rrs cont in remission group and attack group were higher than those in control group, and those in attack group were higher than those in remission group (P<0.05). The relative expression of miR-582-5p, Grs cont and Dmin in remission group and attack group were lower than those in control group, and those in attack group were lower than those in remission group(P<0.05). The expression of serum lncRNA NNT-AS1 in bronchial asthma children was positively correlated with IL-7, TNF-α and Rrs cont (r>0, P<0.05), and negatively correlated with Grs cont and Dmin (r<0, P<0.05). The expression of serum miR-582-5p was negatively correlated with IL-17, TNF-α and Rrs cont (r<0, P<0.05), and positively correlated with Grs cont and Dmin(r>0, P<0.05). Conclusion: Serum lncRNA NNT-AS1 is highly expressed and miR-582-5p is lowly expressed in children with bronchial asthma, the expression of both are closely related to asthma stage, airway inflammation and airway hyperresponsiveness. |
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