| 朱 璐,斯依提·阿木提,郭浩强,张珍连,马丽娟,姚巧玲.慢性间歇性缺氧诱导海马组织炎症致小鼠短期记忆损伤[J].,2024,(21):4047-4053 |
| 慢性间歇性缺氧诱导海马组织炎症致小鼠短期记忆损伤 |
| Chronic Intermittent Hypoxia Induces Short-term Memory Impairment by the Hippocampal Neuroinflammation in Mouse |
| 投稿时间：2024-06-05 修订日期：2024-07-08 |
| DOI：10.13241/j.cnki.pmb.2024.21.008 |
| 中文关键词: 阻塞型睡眠呼吸暂停综合征 慢性间歇性缺氧 短期记忆 海马 星形胶质细胞 炎症 |
| 英文关键词: Obstructive sleep apnea hypopnea syndrome Chronic intermittent hypoxia Short- term memory Hippocampus Astrocytes Inflammation |
| 基金项目:新疆维吾尔自治区自然科学基金项目（2020D01C179） |
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| 中文摘要: |
| 摘要 目的：探讨慢性间歇性缺氧（CIH）对雄性小鼠短期记忆的影响及其可能机制。方法：8周龄雄性C57BL/6小鼠随机分为正常对照组（RA）和间歇性缺氧组（CIH）。RA组小鼠不予任何处理，常规饲养；9 a.m.-9 p.m.将CIH组小鼠放入缺氧箱，给与间歇性缺氧刺激，氧分压在7%-21%范围内循环，每分钟为一个循环，每小时60个循环，12 h/天，为期4周。实验前后测量小鼠体重，通过旷场试验（OFT）和新物体识别试验（NORT）分别检测小鼠自主运动能力和小鼠短期记忆水平；尼氏染色观察小鼠海马组织神经元变化；免疫荧光染色检测小鼠海马CA1区星形胶质细胞激活情况；qRT-PCR检测小鼠海马组织GFAP变化；Elisa检测小鼠海马组织TNFα，IL-6，IL-1β水平。结果：间歇性缺氧4周，RA组小鼠平均体重明显高于CIH组，差异有统计学意义（P=0.002）；OFT结果显示，RA和CIH组小鼠总运动路程（P=0.3156）和运动速度无明显差异（P=0.3157）；NORT训练期两组小鼠识别指数无明显差异（P=0.1596），NORT测试期CIH小鼠识别指数显著低于RA组（P=0.0063）；尼氏染色显示CIH小鼠海马区神经元稀疏，尼氏体着色淡；免疫荧光染色显示CIH组小鼠海马CA1区GFAP阳性面积高于RA组（P=0.0223）； qRT-PCR结果显示CIH组小鼠海马GFAP表达显著高于RA组（P=0.0175）；Elisa检测结果显示与RA组相比，CIH小鼠海马组织TNFα（P=0.02），IL-6（P=0.0492），IL-1β（P=0.0473）水平明显增高。结论：慢性间歇性缺氧4周可能通过诱导海马区炎症致小鼠短期记忆损伤。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of Chronic intermittent hypoxia on the short-term memory and possible mechanisms involved. Methods: The 8 weeks male C57BL/6 mouse were randomly divided into Room Air (RA) and Chronic intermittent hypoxia(CIH) group. The CIH mice were exposed to CIH four weeks during the light phase (9 a.m.-9 p.m.). Measure the body weight of mice before and after the CIH; Open field test (OFT) and new object recognition tests (NORT) were used to assess autonomous motor ability and short-term memory of mice respond to CIH after four weeks, respectively. Hippocampus tissues were obtained to observe the neuron changes through Nissl Staining; Immunofluorescence staining and qRT-PCR were used to detect the activation of astrocytes in hippocampus tissue of mouse; Elisa detected changes in TNFα, IL-6, and IL-1β in mouse hippocampus tissue. Results: After 4 weeks of intermittent hypoxia, the average body weight of the RA group mice was significantly higher than that of the CIH group, and the difference was statistically significant (P=0.002); The results of the OFT showed that there was no significant difference in total exercise distance (P=0.3156) and velocity between the RA and CIH groups of mice (P=0.3157); There was no significant difference in the recognition index between the two groups of mice during the training period of the NORT test (P=0.1596). During the testing period of the NORT test, CIH mice spent less time exploring new objects than RA mice, and the recognition index of the CIH group was significantly lower than that of the RA group (P=0.0063); Nissl staining showed that neurons in the hippocampus CA1 of CIH mice were sparse and Nissl bodies were pale; Immunofluorescence staining showed that the relative GFAP positive area in the hippocampus CA1 region of CIH mice was higher than that in the RA group(P=0.0223); qRT-PCR showed that the expression of GFAP in the hippocampus of CIH group mice was significantly higher than that of RA group mice (P=0.0175); The levels of TNFα (P=0.02), IL-6 (P=0.0492), and IL-1β (P=0. 0473) in the hippocampus of CIH mice increased compared to the RA group by Elisa. Conclusion: Chronic intermittent hypoxia for 4 weeks may induce short-term memory impairment in mice by inducing hippocampus inflammation. |
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