| 希林古丽·吾守尔,库热西·玉努斯,卡思木江·阿西木江,纳菲沙·卡德尔,牟海燕.药物干预PKC-NF-κB通路治疗溃疡性结肠炎的机制研究[J].,2024,(21):4035-4040 |
| 药物干预PKC-NF-κB通路治疗溃疡性结肠炎的机制研究 |
| The Mechanism of UC Intervention on PKC-NF-κB Pathway in the Treatment of Ulcerative Colitis |
| 投稿时间：2024-04-22 修订日期：2024-06-15 |
| DOI：10.13241/j.cnki.pmb.2024.21.006 |
| 中文关键词: 药物干预 PKC-NF-κB通路 溃疡性结肠炎 治疗机制 |
| 英文关键词: Protein kinase C NF-κB Matrix metalloprotein TGF-β1 |
| 基金项目:新疆维吾尔自治区自然科学基金项目（2021D01C249） |
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| 中文摘要: |
| 摘要 目的：研究药物干预PKC-NF-κB通路治疗溃疡性结肠炎的机制。方法：本研究以动物实验研究为主，拟选取33只成年雄性SD大鼠为研究对象，按照研究要求，将SD大鼠分为五组，分别为正常组、低剂量组、中剂量组、高剂量组及UC组，其中，正常组（n=7）、高剂量组（n=6）、中剂量组（n=6）、低剂量组（n=9）、UC组（n=5）。观察五组免疫组化结果、结肠结果、下丘脑结果分析，并进行结肠组织细胞因子相对表达水平，证实药物干预PKC-NF-κB通路治疗溃疡性结肠炎的机制。结果：五组免疫组化检查结果显示，UC组与高剂量组、中剂量组、低剂量组及正常组对比差异有统计学意义（P＜0.05），且五组结肠结果及下丘脑检查结果比较差异有统计学意义（P＜0.05），同时五组结肠组织细胞因子mRNA相对表达水平对比结果显示，UC组、高剂量组、中剂量组、低剂量组各项指标水平高于正常组（P＜0.05），高剂量组各项指标水平高于中剂量组、低剂量组及正常组（P＜0.05），而中剂量组高于低剂量组及正常组（P＜0.05），低剂量组水平高于正常组（P＜0.05）。结论：药物干预PKC-NF-κB通路治疗溃疡性结肠炎效果显著，能够在结肠组织细胞因子mRNA相对表达水平对比分析中也有直接关联，因而可通过药物干预为溃疡性结肠炎治疗提供帮助。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the mechanism of UC intervention on PKC-NF-κB pathway in the treatment of ulcerative colitis. Methods: In this study, 33 adult male SD rats were selected and divided into five groups according to the research requirements, they were divided into normal group, low-dose group, middle-dose group, high-dose group and UC Group, among which, normal group (N=7), high-dose group (N=6), middle-dose group (N=6), low-dose group (N=9) and UC Group (N=5). The immunohistochemical results, colonic results and hypothalamic results were analyzed. The relative expression levels of cytokines in colon tissue were measured to confirm the mechanism of UC intervention on PKC-NF-κB pathway in the treatment of ulcerative colitis. Results: The results of immunohistochemistry showed that there were significant differences between UC Group and high-dose group, middle-dose group, low-dose group and normal group (P<0.05), there were significant differences in the results of colonic examination and hypothalamus examination among the five groups (P<0.05), the indexes of UC Group, high dose group, middle dose group and low dose group were higher than those of normal group (P<0.05). The indexes of high dose group were higher than those of middle dose group, low dose group and normal group (P<0.05), the level in the middle dose group was higher than that in the low dose group and the normal group (P<0.05), and the level in the low dose group was higher than that in the normal group (P<0.05). Conclusion: UC intervention of PKC-NF-κB pathway is effective in the treatment of ulcerative colitis, and can be directly related to the relative expression level of cytokine mRNA in colon tissue, UC intervention can therefore help in the treatment of ulcerative colitis. |
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