| 石 敏,李文娟,周英旎,张 颖,李晓苗.2型糖尿病患者外周血单个核细胞lncRNA VIM-AS1、lncRNA LINC-PINT表达及其临床意义[J].,2024,(20):3884-3886 |
| 2型糖尿病患者外周血单个核细胞lncRNA VIM-AS1、lncRNA LINC-PINT表达及其临床意义 |
| Expression and Clinical Significance of lncRNA VIM-AS1 and lncRNA LINC-PINT in Peripheral Blood Mononuclear Cells of Patients with Type 2 Diabetes |
| 投稿时间：2024-05-16 修订日期：2024-06-10 |
| DOI：10.13241/j.cnki.pmb.2024.20.020 |
| 中文关键词: 2型糖尿病 外周血单个核细胞 lncRNA VIM-AS1 lncRNA LINC-PINT 预测价值 |
| 英文关键词: Type 2 diabetes Peripheral blood mononuclear cells lncRNA VIM-AS1 lncRNA LINC-PINT Predictive value |
| 基金项目:国家自然科学基金面上项目（81573746） |
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| 中文摘要: |
| 摘要 目的：探讨2型糖尿病（T2DM）患者外周血单个核细胞（PBMC）长链非编码核糖核酸（lncRNA）VIM反义RNA1（VIM-AS1）、lncRNA-p53诱导转录本（LINC-PINT）表达及其临床意义。方法：选取120例T2DM患者（T2DM组），另选取同期体检健康的80例志愿者（NC组）。检测并对比两组lncRNA VIM-AS1、lncRNA LINC-PINT表达及糖脂代谢、胰岛素抵抗指标。采用受试者工作特征（ROC）曲线分析lncRNA VIM-AS1、lncRNA LINC-PINT单独及联合预测T2DM发生的价值。结果：T2DM组lncRNA VIM-AS1、lncRNA LINC-PINT表达均低于NC组（P＜0.05）；T2DM组三酰甘油（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、空腹血糖（FPG）、餐后2 h血糖（2hPG）、糖化血红蛋白（HbAlc）、空腹胰岛素（FINS）、胰岛素抵抗指数（HOMA-IR）水平均高于NC组，高密度脂蛋白胆固醇（HDL-C）水平低于NC组（P＜0.05）；绘制ROC曲线发现，lncRNA VIM-AS1、lncRNA LINC-PINT单独及联合预测T2DM发生的曲线下面积（AUC）为0.824、0.746、0.900，联合预测价值高于各指标单独检测。结论：T2DM患者PBMC中lncRNA VIM-AS1、lncRNA LINC-PINT呈低表达，联合检测对T2DM发生具有较高的预测价值。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression and clinical significance of long non-coding RNA (lncRNA) VIM antisense RNA1 (VIM-AS1) and lncRNA-p53 induced transcript (LINC-PINT) in peripheral blood mononuclear cells (PBMC) of patients with type 2 diabetes (T2DM). Methods: 120 patients with T2DM (T2DM group), and 80 volunteers (NC group) were selected during the same period. The expression of lncRNA VIM-AS1, lncRNA LINC-PINT, glucose and lipid metabolism and insulin resistance were detected and compared between two groups. The value of lncRNA VIM-AS1, lncRNA LINC-PINT alone and in combination in predicting the occurrence of T2DM were analyzed by receiver operating characteristic (ROC) curve. Results: The expression of lncRNA VIM-AS1 and lncRNA LINC-PINT in T2DM group was lower than that in NC group (P<0.05). The levels of triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), fasting plasma glucose (FPG), 2 h postprandial blood glucose (2hPG), glycosylated hemoglobin (HbAlc), fasting insulin (FINS) and insulin resistance index (HOMA-IR) in T2DM group were higher than those in NC group, the level of high density lipoprotein cholesterol (HDL-C) was lower than that in NC group (P<0.05). ROC curve showed that, the area under the curve（AUC）of lncRNA VIM-AS1 and lncRNA LINC-PINT alone and in combination to predict the occurrence of T2DM were 0.824, 0.746 and 0.900, respectively, the combined predictive value was higher than that of each index alone. Conclusion: The low expression of lncRNA VIM-AS1 and lncRNA LINC-PINT in PBMC of patients with T2DM. The combined detection has a high predictive value for the occurrence of T2DM. |
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