| 于 洋,邢嘉豪,王 景,滕 林,回雪颖,张海丽.健脾利湿通脉方对深静脉血栓形成大鼠miR-23a及炎性因子影响的研究[J].,2024,(20):3831-3835 |
| 健脾利湿通脉方对深静脉血栓形成大鼠miR-23a及炎性因子影响的研究 |
| The Effects of Jianpi-Lishi-Tongmai Decoction on miR-23a and Inflammatory Factors in Rats with Deep Vein Thrombosis |
| 投稿时间：2024-07-30 修订日期：2024-08-25 |
| DOI：10.13241/j.cnki.pmb.2024.20.005 |
| 中文关键词: 深静脉血栓形成 miR-23a TNF-α IL-6 IL-1β |
| 英文关键词: DVT miR-23a TNF-α IL-6 IL-1β |
| 基金项目:黑龙江省中医药管理局项目（ZHY2020-156）；黑龙江省中医药管理局国医大师学术思想传承项目（GY2022-22)；黑龙江省第二批省级中医临床优秀人才培养项目(黑中医药教函[2022]28号）；中华中医药学会周围血管病分会第三批师承项目 |
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| 中文摘要: |
| 摘要 目的：通过实验观察健脾利湿通脉方对DVT大鼠miR-23a及相关炎性因子的影响。方法：将140只Wistar 大鼠随机分为Blank group 、Sham control、Modelset、Low dose group、Middle dose group、High-dose group和Positive control，每组10只，构建DVT模型。分别于给药后7天、14天测定miR-23a、IL-1β、IL-6及TNF-α含量。结果：1.14 d，与Blank group比较，Modelset大鼠TNF-α、IL-1β、IL-6、及miR-23a表达显著升高（P＜0.05）；与Modelset比较，Low dose group、Middle dose group、High-dose group及Positive control 大鼠上述指标均显著改善（P＜0.05）；在TNF-α组别内，与Positive control比较，Blank group、Sham control、Modelset、Low dose group、High-dose group均有显著性差异（P＜0.05）；在IL-1β组别内，与Positive control比较，Sham control、Modelset、High-dose group均有显著性差异（P＜0.05）；在IL-6组别内，与Positive control比较，Blank group、Sham control、Middle dose group、High-dose group均有显著性差异（P＜0.05）；在miR-23a组别内，与Positive control比较，Blank group、Sham control、Modelset、Low dose group、Middle dose group均有显著性差异（P＜0.05）。2. 7 d与14 d相比，在大鼠IL-1β、IL-6、TNF-α及miR-23a四个组别中，Blank group、Sham control前后无明显统计学差异（P>0.05）；而Modelset、Low dose group、Middle dose group、High dose group、Positive control前后有明显统计学差异（P<0.05)。结论：健脾利湿通脉方通过下调miR-23a及 IL-1β、 IL-6 、TNF-α表达而发挥治疗作用。 |
| 英文摘要: |
| ABSTRACT Objective: To experimentally observe the effects of Strengthening the Spleen, Promoting Dampness and Channeling the Veins Formula on miR-23a and related inflammatory factors in DVT rats. Methods: 140 Wistar rats were randomly divided into Blank group, Sham control, Modelset, Low dose group, Middle dose group, High-dose group and Positive control, 10 rats in each group, and DVT model was constructed. The contents of miR-23a, IL-1β, IL-6 and TNF-α were measured at 7 and 14 days after drug administration, respectively. Results: 1. At 14 d, the expression of TNF-α, IL-1β, IL-6, and miR-23a was significantly elevated in Modelset rats compared with that in Blank group (P<0.05); the expression of the above indexes was significantly elevated in Low dose group, Middle dose group, High-dose group, and Positive control rats compared with that in Modelset. Positive control rats showed significant improvement in the above indexes (P<0.05); within the TNF-α group, Blank group, Sham control, Modelset, Low dose group, High-dose group showed significant differences compared with Positive control (P<0.05); within the IL-1β group, there was a significant difference between Sham control, Modelset, and High-dose group when compared with Positive control (P<0.05); within the IL-6 group, Blank group when compared with Positive control, Sham control, Middle dose group, and High-dose group were all significantly different (P<0.05); within the miR-23a group, compared with Positive control, Blank group, Sham control, Modelset, Low dose group, and Middle dose group were significantly different (P<0.05). 2. In the four groups of IL-1β, IL-6, TNF-α, and miR-23a in rats at 7 d compared with 14 d, there were no statistically significant differences before and after the Blank group, and the Sham control (P>0.05); and the Modelset, Low dose group, Middle dose group, High dose group, Positive control before and after had significant statistical differences (P<0.05). Conclusion: Strengthening the spleen, inducing dampness and promoting circulation formula exerted therapeutic effects by down-regulating miR-23a and the expression of IL-1β, IL-6, and TNF-α. |
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