| 范静华,王彦华,杨艳娥,徐向龄,林 源.PCIS评分联合血清microRNA-125b、G-CSF在病毒性脑炎患儿中的表达情况及其与预后的相关性分析[J].,2024,(18):3437-3441 |
| PCIS评分联合血清microRNA-125b、G-CSF在病毒性脑炎患儿中的表达情况及其与预后的相关性分析 |
| Expression of PCIS Score Combined with Serum MicroRNA-125b and G-CSF in Children with Viral Encephalitis and Their Correlation with Prognosi |
| 投稿时间：2024-04-12 修订日期：2024-05-05 |
| DOI：10.13241/j.cnki.pmb.2024.18.006 |
| 中文关键词: 小儿 病毒性脑炎 小儿危重病例评分法评分 microRNA-125b 粒细胞集落刺激因子 预后 |
| 英文关键词: Children Viral encephalitis Pediatric critical case scoring method microRNA-125b Granulocyte colony-stimulating factor Prognosis |
| 基金项目:陕西省自然科学基础研究计划项目（2023-JC-ZD-55） |
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| 中文摘要: |
| 摘要 目的：分析小儿危重病例评分法（PCIS）评分联合血清microRNA-125b、粒细胞集落刺激因子（G-CSF）在病毒性脑炎患儿中的表达情况及其与预后的相关性。方法：选择我院自2022年1月至2023年6月收治的105例病毒性脑炎患儿纳入观察组，另选同期的105例健康体检儿纳入对照组。所有入选者均进行PCIS评分，检测血清microRNA-125b、G-CSF表达水平，分析PCIS评分、血清microRNA-125b、G-CSF表达水平在不同严重程度的病毒性脑炎患儿中的差异性及与脑脊液中脑损伤指标的关系；随访6个月，记录预后不良情况，使用多因素Logistic回归和受试者工作特征（ROC）曲线分析PCIS评分、血清microRNA-125b、G-CSF与预后不良的关系。结果：观察组PCIS评分低于对照组，血清microRNA-125b、G-CSF表达水平均高于对照组（P＜0.05）；在105例病毒性脑炎患儿中，重症40例、非重症65例；重症组PCIS评分低于非重症组，血清microRNA-125b、G-CSF表达水平均高于非重症组（P＜0.05）；经Pearson相关性分析，病毒性脑炎患儿PCIS评分、血清microRNA-125b、G-CSF表达水平与脑脊液中髓鞘碱性蛋白（MBP）、脑型肌酸激酶（CK-BB）、神经元特异性烯醇化酶（NSE）表达水平均呈正相关（P＜0.05）；经多因素Logistic回归，PCIS评分、血清microRNA-125b、G-CSF均是病毒性脑炎患儿预后不良的独立预测因素（P＜0.05）；经ROC曲线分析，PCIS评分、血清microRNA-125b联合G-CSF预测病毒性脑炎患儿预后不良的AUC为0.923。结论：PCIS评分、血清microRNA-125b、G-CSF均与病毒性脑炎患儿病情严重程度有关，联合应用可提高对预后不良的预测水平。 |
| 英文摘要: |
| ABSTRACT Objective: To analyze the expression of pediatric Critical Case scoring (PCIS) combined with serum microRNA-125b and granulocyte colony stimulating factor (G-CSF) in children with viral encephalitis and its correlation with prognosis. Methods: 105 children with viral encephalitis admitted to our hospital from January 2022 to June 2023 were selected to be included in the observation group, and another 105 healthy physical examination children in the same period were selected to be included in the control group. All enrollees were subjected to PCIS scoring, and serum microRNA-125b and G-CSF expression levels were detected to analyze the variability of PCIS scoring, serum microRNA-125b, and G-CSF expression levels in children with viral encephalitis of different severity and the relationship with indicators of cerebral damage in the cerebrospinal fluid; with a follow-up of 6 months, the poor prognosis was recorded, and a multifactorial logistic regression and a multifactor logistic regression were used to measure the prognosis. Factor logistic regression and subject work characteristics (ROC) curves were used to analyze the relationship between PCIS score, serum microRNA-125b, G-CSF and poor prognosis. Results: The PCIS score of observation group was lower than that of control group, and the expression levels of serum microRNA-125b and G-CSF were higher than those of control group (P<0.05). Of the 105 children with viral encephalitis, 40 were severe and 65 were non-severe cases. The PCIS score of the critical group was lower than that of the non-critical group, and the expression levels of serum microRNA-125b and G-CSF were higher than those of the non-critical group (P<0.05). By Pearson correlation analysis, the children with viral encephalitis PCIS score, serum microRNA-125b, the expression level of G-CSF and myelin basic protein (MBP) in cerebrospinal fluid, brain type of creatine kinase (CK-BB), neuron specific enolization enzyme (NSE) expression levels were positively correlated (P<0.05). The multi-factor Logistic regression, PCIS score, serum microRNA-125b and G-CSF is children with viral encephalitis is an independent predictor of poor prognosis (P<0.05). The ROC curve analysis, PCIS score, serum microRNA-125b joint G-CSF prediction of prognosis of children with viral encephalitis, AUC is 0.923. Conclusion: PCIS score, serum microRNA-125b, and G-CSF are all associated with severity of illness in children with viral encephalitis, and the combination improves the level of prediction of poor prognosis. |
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