| 林俊平,李少珊,张 立,曹金涛,马 涛.桥本甲状腺炎患者外周血miR-142-3p、miR-125a-5p水平与甲状腺功能和Th1/Th2及Th17/Treg细胞平衡的关系[J].,2024,(17):3269-3273 |
| 桥本甲状腺炎患者外周血miR-142-3p、miR-125a-5p水平与甲状腺功能和Th1/Th2及Th17/Treg细胞平衡的关系 |
| Relationship between Peripheral Blood miR-142-3p, miR-125a-5p Levels and Thyroid Function, Th1/Th2 and Th17/Treg Cell Balance in Patients with Hashimoto's Thyroiditis |
| 投稿时间：2024-03-23 修订日期：2024-04-18 |
| DOI：10.13241/j.cnki.pmb.2024.17.014 |
| 中文关键词: 桥本甲状腺炎 miR-142-3p miR-125a-5p 辅助性T细胞1/辅助性T细胞2 辅助性T细胞17/调节性T细胞 甲状腺功能 |
| 英文关键词: Hashimoto's thyroiditis miR-142-3p miR-125a-5p T helper cells 1/T helper cells 2 T helper cells 17/T regulatory cells Thyroid function |
| 基金项目:山东省科研基金项目（2021kh0311） |
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| 中文摘要: |
| 摘要 目的：探讨桥本甲状腺炎（HT）患者外周血微小核糖核酸（miRNA）-142-3p、miR-125a-5p水平与甲状腺功能和辅助性T细胞（Th）1/Th2及Th17/调节性T细胞（Treg）细胞平衡的关系。方法：选取2020年1月～2023年8月我院收治的HT患者90例作为HT组和同时间段90名体检健康者作为对照组，根据甲状腺功能减低程度将HT患者分为甲状腺功能正常组（26例）、亚临床甲状腺功能减退组（31例）和临床甲状腺功能减退组（33例）。采用实时荧光定量聚合酶链式反应检测外周血miR-142-3p、miR-125a-5p水平，酶联免疫吸附法检测甲状腺功能指标[甲状腺球蛋白抗体（TgAb）、甲状腺过氧化物酶抗体（TPOAb）、促甲状腺激素（TSH）、游离三碘甲状腺原氨酸（FT3）、游离甲状腺素（FT4）]，流式细胞术检测外周血Th1、Th2、Th17、Treg细胞比例，并计算Th1/Th2及Th17/Treg比值。通过Pearson/Spearman相关性分析HT患者外周血miR-142-3p、miR-125a-5p与甲状腺功能、Th1、Th2、Th17、Treg的相关性。结果：HT组外周血miR-142-3p、miR-125a-5p、TgAb、TPOAb、TSH、Th1、Th17、Th1/Th2、Th17/Treg比值高于对照组，FT3、FT4、Th2、Treg比例低于对照组（P＜0.05）。甲状腺功能正常组、亚临床甲状腺功能减退组、临床甲状腺功能减退组外周血miR-142-3p、miR-125a-5p、TgAb、TPOAb、TSH、Th1、Th17、Th1/Th2、Th17/Treg比值依次升高，FT3、FT4、Th2、Treg比例依次降低（P＜0.05）。Pearson/Spearman相关性分析显示，HT患者外周血miR-142-3p、miR-125a-5p与TgAb、TPOAb、TSH、Th1、Th17、Th1/Th2、Th17/Treg呈正相关，与FT3、FT4、Th2、Treg呈负相关（P＜0.05）。结论：HT患者外周血miR-142-3p、miR-125a-5p水平升高，与甲状腺功能减退和Th1/Th2、Th17/Treg细胞失衡有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between peripheral blood microribonucleic acid (miRNA)-142-3p, miR-125a-5p levels and thyroid function and T helper cells (Th)1/Th2 and Th17/T regulatory cell (Treg) cells balance in patients with Hashimoto's thyroiditis (HT). Methods: 90 HT patients admitted to our hospital from January 2020 to August 2023 were selected as HT group and 90 healthy people in the same period were selected as control group, HT patients were divided into normal thyroid function group (26 cases), subclinical hypothyroidism group (31 cases) and clinical hypothyroidism group (33 cases) according to the degree of hypothyroidism. The levels of miR-142-3p and miR-125a-5p in peripheral blood were detected by real-time fluorescence quantitative polymerase chain reaction, thyroid function indexes [thyroglobulin antibody (TgAb), thyroid peroxidase antibody (TPOAb), thyroid stimulating hormone (TSH), free triiodothyronine (FT3), free thyroxine (FT4)] were detected by enzyme-linked immunosorbent assay, the proportions of Th1, Th2, Th17 and Treg cells in peripheral blood were detected by flow cytometry, and the ratios of Th1/Th2 and Th17/Treg were calculated. The correlation between miR-142-3p, miR-125a-5p and thyroid function, Th1, Th2, Th17, Treg in peripheral blood of HT patients was analyzed by Pearson/Spearman correlation analysis. Results: The ratios of miR-142-3p, miR-125a-5p, TgAb, TPOAb, TSH, Th1, Th17, Th1/Th2 and Th17/Treg in peripheral blood in HT group were higher than those in control group, while the ratios of FT3, FT4, Th2 and Treg were lower than those in control group (P<0.05). The ratios of miR-142-3p, miR-125a-5p, TgAb, TPOAb, TSH, Th1, Th17, Th1/Th2 and Th17/Treg in peripheral blood in normal thyroid function group, subclinical hypothyroidism group and clinical hypothyroidism group increased in turn, while the ratios of FT3, FT4, Th2 and Treg decreased in turn (P<0.05). Pearson/Spearman correlation analysis showed that, miR-142-3p and miR-125a-5p in peripheral blood of HT patients were positively correlated with TgAb, TPOAb, TSH, Th1, Th17, Th1/Th2 and Th17/Treg, and negatively correlated with FT3, FT4, Th2 and Treg (P<0.05). Conclusion: The levels of miR-142-3p and miR-125a-5p in peripheral blood of HT patients are increase, which are relate to hypothyroidism and imbalance of Th1/Th2 and Th17/Treg cells. |
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