| 范 蕾,何 萱,茅洪维,张评奥,张永芳.异菝葜皂苷元对MPP+诱导的SH-SY5Y细胞凋亡的影响[J].,2024,(14):2601-2605 |
| 异菝葜皂苷元对MPP+诱导的SH-SY5Y细胞凋亡的影响 |
| Protective Effect of Smilagenin on MPP+-induced Apoptosis in SH-SY5Y Cells |
| 投稿时间：2024-01-26 修订日期：2024-02-23 |
| DOI：10.13241/j.cnki.pmb.2024.14.001 |
| 中文关键词: 异菝葜皂苷元 中草药 SH-SY5Y 细胞凋亡 帕金森病 |
| 英文关键词: Smilagenin Herbal medicine SH-SY5Y Apoptosis Parkinson's disease |
| 基金项目:国家自然科学基金项目（82003719） |
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| 中文摘要: |
| 摘要 目的：探讨菝葜皂苷元对MPP+诱导的SH-SY5Y细胞凋亡的影响及机制。方法：采用MPP+处理人神经母细胞瘤SH-SY5Y细胞构建体外PD细胞模型。取对数生长期的SH-SY5Y细胞分为四组：Control组（正常对照组），MPP+组(模型组)，异菝葜皂苷元组（10 μM），MPP++异菝葜皂苷元组（3 mM, 10 μM)。首先采用细胞增殖实验（CCK-8）检测异菝葜皂苷元作用的最佳浓度及对SH-SY5Y细胞活力的影响。采用AnnexinV-FITC/PI双染法检测异菝葜皂苷元对SH-SY5Y细胞凋亡率的影响，采用蛋白免疫印迹实验检测凋亡相关蛋白Bax, Bcl-2的蛋白表达。结果：细胞活力实验确定10 μM/mL的异菝葜皂苷元作为后续试验的添加浓度。与对照组相比，MPP+组细胞活力显著降低（P＜0.001），细胞凋亡率显著升高（P＜0.001），凋亡相关蛋白Bax/Bcl-2表达显著升高（P＜0.01）。相较于MPP+组，MPP++异菝葜皂苷元组细胞活力显著升高（P＜0.001），细胞凋亡率显著降低（P＜0.01），凋亡相关蛋白Bax/Bcl-2表达显著升高降低（P＜0.01）。结论：异菝葜皂苷元可以抑制MPP+诱导的SH-SY5Y细胞凋亡，可能与其调节抗凋亡相关蛋白Bax, Bcl-2的蛋白表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: The purpose of this article was to investigate the exerting protective effect of smilagenin on MPP+-induced apoptosis in SH-SY5Y cells. Methods: In the experiment, PD cell model was constructed using MPP+ treated human neuroblastoma SH-SY5Y cells. SH-SY5Y cells in logarithmic growth phase were taken and divided into four groups: the Control group (Control), the MPP+ group (model group), the smilagenin group (10 μM), and the MPP++ smilagenin group (3 mm,10 μM). The cell proliferation assay (CCK-8) was firstly used to detect the optimal concentration of smilagenin action and the effect on SH-SY5Y cell viability. The effect of smilagenin on the apoptosis rate of SH-SY5Y cells was detected by AnnexinV-FITC/PI double staining assay, and the protein expression of apoptosis-related proteins Bax, Bcl-2 was detected by protein immunoblotting assay. Results: The cell viability assay determined 10 μM/mL of smilagenin as the added concentration for the subsequent experiments. Compared with the control group, the MPP+ group showed significantly lower cell viability (P＜0.001), higher apoptosis rate(P＜0.001), and significantly higher expression of apoptosis-related protein Bax/Bcl-2(P＜0.01). Compared with the MPP+ group, cell viability was significantly higher in the MPP++ smilagenin group (P<0.001), apoptosis rate was significantly lower(P<0.01), apoptosis-related protein Bax/Bcl-2 expression was significantly lower (P<0.01). Conclusion: Smilagenin can inhibit MPP+-induced apoptosis in SH-SY5Y cells, which may be related to its regulation of protein expression of apoptosis-related proteins Bax and Bcl-2. |
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