| 邱丽君,张静怡,袁健珊,马晴晴,葛 格,王云洁.宫颈鳞状细胞癌组织LncRNA DCST1-AS1、LncRNA MCM3AP-AS1表达与增殖侵袭基因和预后的关系[J].,2024,(10):1985-1990 |
| 宫颈鳞状细胞癌组织LncRNA DCST1-AS1、LncRNA MCM3AP-AS1表达与增殖侵袭基因和预后的关系 |
| Relationship between the Expression of LncRNA DCST1-AS1 and LncRNA MCM3AP-AS1 in Cervical Squamous Cell Carcinoma Tissue and the Proliferation and Invasion Genes and Prognosis |
| 投稿时间：2023-11-26 修订日期：2023-12-23 |
| DOI：10.13241/j.cnki.pmb.2024.10.038 |
| 中文关键词: 宫颈鳞状细胞癌 LncRNA DCST1-AS1 LncRNA MCM3AP-AS1 增殖侵袭基因 预后 |
| 英文关键词: Cervical squamous cell carcinoma LncRNA DCST1-AS1 LncRNA MCM3AP-AS1 Proliferation and invasion gene Prognosis |
| 基金项目:江苏省妇幼健康重点人才项目（FRC201714） |
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| 摘要点击次数: 215 |
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| 中文摘要: |
| 摘要 目的：探讨宫颈鳞状细胞癌（CSCC）组织长链非编码核糖核酸（LncRNA）DC-STAMP结构域1-反义1（DCST1-AS1）、LncRNA MCM3AP反义RNA 1（MCM3AP-AS1）表达与增殖侵袭基因和预后的关系。方法：选择2018年1月至2020年1月苏州大学第二附属医院收治的124例CSCC患者，取CSCC组织及其癌旁正常组织，实时荧光定量聚合酶链反应（qRT-PCR）检测LncRNA DCST1-AS1、LncRNA MCM3AP-AS1以及增殖基因（YAP1、Piwil2、EZH2、PKCε）和侵袭基因（Rab11、TUG1、Gli1、FoxM1）mRNA表达。患者出院后随访3年。Pearson相关性分析Lnc RNA DCST1-AS1、Lnc RNA MCM3AP-AS1表达与增殖基因、侵袭基因的相关性。分析Lnc RNA DCST1-AS1、Lnc RNA MCM3AP-AS1表达与临床病理特征的关系。绘制Kaplan-Meier曲线分析预后情况，多因素COX回归分析影响CSCC患者预后的因素。结果：CSCC癌组织中Lnc RNA DCST1-AS1表达高于癌旁组织（P＜0.05），Lnc RNA MCM3AP-AS1表达低于癌旁组织（P＜0.05）。YAP1、Piwil2、EZH2、PKCε、Rab11、TUG1、Gli1、FoxM1 mRNA水平与CSCC癌组织中Lnc RNA DCST1-AS1表达呈正相关（P＜0.05），与Lnc RNA MCM3AP-AS1呈负相关（P＜0.05）。低中度分化、FIGO分期Ⅲa期、盆腔淋巴结转移CSCC患者癌组织中Lnc RNA DCST1-AS1表达高于高度分化、FIGO分期Ⅰ～Ⅱ期、无盆腔淋巴结转移宫颈癌患者（P＜0.05），Lnc RNA MCM3AP-AS1表达低于高度分化、FIGO分期Ⅰ～Ⅱ期、无盆腔淋巴结转移宫颈癌患者（P＜0.05）。Lnc RNA DCST1-AS1高表达、Lnc RNA MCM3AP-AS1低表达CSCC患者3年总生存率低于Lnc RNA DCST1-AS1低表达、Lnc RNA MCM3AP-AS1高表达患者（P＜0.05）。多因素COX回归分析显示盆腔淋巴结转移、Lnc RNA DCST1-AS1高表达是CSCC患者预后不良的危险因素（P＜0.05），Lnc RNA MCM3AP-AS1高表达是保护因素（P＜0.05）。结论：CSCC组织中LncRNA DCST1-AS1表达上调，LncRNA MCM3AP-AS1表达下调，且与CSCC增殖侵袭和预后不良有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between the expression of long non-coding ribonucleic acids (LncRNA) DC-STAMP domain 1-antisense 1 (DCST1-AS1) and LncRNA MCM3AP antisense RNA 1 (MCM3AP-AS1) in cervical squamous cell carcinoma (CSCC) tissue and the proliferation and invasion genes and prognosis. Methods: 124 CSCC patients admitted to The Second Affiliated Hospital of Soochow University from January 2018 to January 2020 were selected, CSCC tissues and cancer adjacent normal tissues were taken, the mRNA expression of LncRNA DCST1-AS1, LncRNA MCM3AP-AS1, proliferation genes (YAP1, Piwil2, EZH2, PKCε) and invasion genes (Rab11, TUG1, Gli1, FoxM1) were detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR). Patients were followed up for 3 years after discharge. The correlation between Lnc RNA DCST1-AS1, Lnc RNA MCM3AP-AS1 expression and proliferation genes and invasion genes were analyzed by Pearson correlation analysis. Analyzed the relationship between the expression of Lnc RNA DCST1-AS1, Lnc RNA MCM3AP-AS1 and clinical pathological features. The prognosis was analyzed by plotted Kaplan-Meier curve, and the factors affecting the prognosis of CSCC patients were analyzed by multivariate COX regression analysis. Results: The expression of Lnc RNA DCST1-AS1 in CSCC cancer tissues was higher than that in cancer adjacent tissues (P<0.05), and the expression of Lnc RNA MCM3 AP-AS1 was lower than that in cancer adjacent tissues (P<0.05). The mRNA levels of YAP1, Piwil2, EZH2, PKCε, Rab11, TUG1, Gli1 and FoxM1 were positively correlated with the expression of Lnc RNA DCST1-AS1 in CSCC tissues (P<0.05), and negatively correlated with Lnc RNA MCM3AP-AS1 (P<0.05). The expression of Lnc RNA DCST1-AS1 in cancer tissues of CSCC patients with low-moderate differentiation, FIGO stage IIIa and pelvic lymph node metastasis was higher than that in patients with high differentiation, FIGO stage I～II and no pelvic lymph node metastasis (P<0.05), the expression of Lnc RNA MCM3 AP-AS1 was lower than that in patients with high differentiation, FIGO stage I～II and no pelvic lymph node metastasis (P<0.05). The 3-year overall survival rate of CSCC patients with high expression of Lnc RNA DCST1-AS1 and low expression of Lnc RNA MCM3AP-AS1 was lower than that of low expression of Lnc RNA DCST1-AS1 and high expression of Lnc RNA MCM3AP-AS1 (P<0.05). Multivariate COX regression analysis showed that, pelvic lymph node metastasis and high expression of Lnc RNA DCST1-AS1 were risk factors for poor prognosis in patients with CSCC (P<0.05), and high expression of Lnc RNA MCM3AP-AS1 was a protective factor(P<0.05). Conclusion: The expression of LncRNA DCST1-AS1 is up-regulate and the expression of LncRNA MCM3AP-AS1 is down-regulate in CSCC tissues, which is relate to the proliferation, invasion and poor prognosis of CSCC. |
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