| 白 冰,阿瓦汉·吐尔迪,古丽仙·吐尔逊,热仙古丽·买买提,阿孜尔古丽·阿布都克日木,孙玉萍.阴道菌群多样性与宫颈癌的相关性研究[J].,2024,(9):1686-1691 |
| 阴道菌群多样性与宫颈癌的相关性研究 |
| A study on the Correlation between Vaginal Flora Diversity and Cervical Cancer |
| 投稿时间：2023-12-04 修订日期：2023-12-28 |
| DOI：10.13241/j.cnki.pmb.2024.09.017 |
| 中文关键词: 阴道菌群 宫颈癌 乳酸杆菌 |
| 英文关键词: Vaginal flora Cervical cancer Lactobacillus |
| 基金项目:新疆维吾尔自治区自然科学基金项目（2022D01C185） |
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| 中文摘要: |
| 摘要 目的：研究宫颈癌疾病中女性阴道菌群的构成变化，探索具有预防宫颈癌发生的阴道益生菌。方法：选取新疆医科大学附属肿瘤医院和社区募集的24例健康女性和43例宫颈癌女性。采用16S rDNA和宏基因组测序技术分析其阴道菌群构成。结果：两组女性阴道菌群在chao1指数和ASV number中无统计学差异，提示群落丰度相似，在表示物种多样性的Shannon指数和Simpson指数中宫颈癌组显著增高，差异具有统计学意义。在微生物分类的门水平，两组阴道微生物均以厚壁菌门为主要物种，但宫颈癌组（45%）较健康组（91%）比例有所下降，其余如变形菌门和放线菌门等所占比例均显著升高。在种水平，宫颈癌组中的乳酸杆菌属（14.2%）较健康组（81%）比例下降明显，在宏基因组测序基础上，利用LEfSe及LDA判别分析得出在健康女性中卷曲乳杆菌具有益生菌特征意义，并可能在ABC转运体功能中发挥益生效应。结论：宫颈癌女性具有阴道微生物多样性增加，乳酸杆菌水平减少的特点，其中，通过宏基因组测序技术探究微生物种水平的差异物种得出卷曲乳杆菌在ABC转运体功能中具有潜在预防宫颈癌发生的益生菌作用，可为今后临床在调节阴道微生态角度预防宫颈癌发生提供理论依据。 |
| 英文摘要: |
| ABSTRACT Objective: To study the changes in the composition of vaginal flora in women with cervical cancer disease and to explore vaginal probiotics with the potential to prevent the development of cervical cancer. Methods: Twenty-four healthy women and 43 women with cervical cancer were selected from the Cancer Hospital of Xinjiang Medical University and community collections. Their vaginal flora composition was analysed using 16S rDNA and macro-genome sequencing techniques. Results: There was no statistically significant difference between the vaginal flora of the two groups of women in chao1 index and ASV number, suggesting a similar community abundance, while in Shannon and Simpson indices, which indicate species diversity, the cervical cancer group showed a significant increase, and the difference was statistically significant. At the phylum level of microbial classification, the thick-walled phylum was the main species of vaginal microorganisms in both groups, but the proportion of the cervical cancer group (45%) decreased compared with that of the healthy group (91%), while the proportion of the rest, such as Ascomycetes and Actinomycetes, was significantly higher. At the species level, the proportion of Lactobacillus spp. in the cervical cancer group (14.2%) decreased significantly compared to that in the healthy group (81%). On the basis of macro-genome sequencing, it was concluded that Lactobacillus convolvulus has a probiotic signature significance in healthy females and may exert a probiotic effect in the function of the ABC transporter, using LEfSe and LDA discriminant analysis. Conclusion: Women with cervical cancer are characterised by an increase in vaginal microbial diversity and a decrease in the level of Lactobacillus, in which the different species at the level of microbial species was explored by macro-genome sequencing technology to conclude that Lactobacillus convolvulus has a potential probiotic role in the function of the ABC transporter in the prevention of the development of cervical cancer, and this can provide theoretical evidence for the prevention of cervical carcinogenesis from the perspective of regulating the vaginal micro-ecology for the future clinic. |
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