董玉杰,王晓景,阮国然,任浩进,陈诗阳,黄海东,张美春.下调miR-223表达对脓毒症心肌病小鼠心肌的保护作用及机制研究[J].,2024,(2):234-239 |
下调miR-223表达对脓毒症心肌病小鼠心肌的保护作用及机制研究 |
Protective Effect and Mechanism of Down-regulation of miR-223 Expression on Myocardium of Septic Cardiomyopathy Mice |
投稿时间:2023-07-13 修订日期:2023-08-08 |
DOI:10.13241/j.cnki.pmb.2024.02.006 |
中文关键词: 脓毒症 脓毒症心肌病 miR-223 miRNA 炎症反应 |
英文关键词: Sepsis Septic cardiomyopathy miR-223 miRNA Inflammatory response |
基金项目:湖北省自然科学基金面上项目(2019CFB621);武汉市医学科研项目(WX20Q27) |
|
摘要点击次数: 428 |
全文下载次数: 224 |
中文摘要: |
摘要 目的:探讨下调miR-223表达对脓毒症心肌病(SCM)小鼠心肌的保护作用及其机制。方法:按随机数字表法将27只8-10 周龄SPF级雄性C57BL/6小鼠分配至SCM模型(0 h,6 h,12 h,18 h,24 h)时相组、Normal组、SCM组、miR-223 antagomir NC组、miR-223 antagomir组,每组3只。腹腔注射脂多糖(lipopolysaccharide, LPS)15 mg/kg构建SCM小鼠模型。miR-223 antagomir NC组与miR-223 antagomir组分别于建模前连续3天鼠尾静脉注射miR-223 antagomir NC、miR-223 antagomir预处理。采用反转录-聚合酶链反应(RT-PCR)研究SCM模型各个时相组小鼠心肌组织miR-223的表达情况。采用苏木素伊红(HE)染色法观察Normal组、SCM组、miR-223 antagomir NC组和miR-223 antagomir组小鼠心肌病理形态变化。采用酶联免疫吸附实验(ELISA)测定Normal组、SCM组、miR-223 antagomir NC组和miR-223 antagomir组小鼠血清cTnI、BNP、CK-MB、IL-6、IL-1β、TNF-α的含量并进行相关性分析。结果:SCM模型时相组小鼠随刺激时间延长,心肌组织miR-223表达水平逐渐升高。与Normal组比较,SCM组、miR-223 antagomir NC组、miR-223 antagomir组小鼠心肌组织出现不同程度损伤;血清心肌损伤标记物cTnI、BNP、CK-MB及炎性因子IL-6、IL-1β、TNF-α的表达水平均上升,差异具有统计学意义(P<0.05);与SCM组比较,miR-223 antagomir NC组各项指标相差不大,差异均无统计学意义(P>0.05);miR-223 antagomir组小鼠心肌组织病理损伤程度有所减轻,心肌损伤标记物cTnI、BNP、CK-MB及炎性因子IL-6、IL-1β、TNF-α水平下降,差异具有统计学意义(P<0.05)。相关性分析结果显示小鼠miR-223表达与心肌损伤标记物cTnI、BNP、CK-MB及炎性因子IL-6、IL-1β、TNF-α的表达呈正相关。结论:下调miR-223表达可通过减轻炎症反应对SCM小鼠心肌产生保护作用。 |
英文摘要: |
ABSTRACT Objective: Investigating the protective effect and mechanisms of downregulating miR-223 expression on the myocardium of mice with septic cardiomyopathy (SCM). Methods: Using a random number table, 27 male SPF C57BL/6 mice aged 8-10 weeks were allocated into different groups: SCM model groups at different time points (0 h, 6 h, 12 h, 18 h, 24 h), Normal group, SCM group, miR-223 antagomir NC group, and miR-223 antagomir group, with 3 mice in each group. The SCM mouse model was established by intraperitoneal injection of lipopolysaccharide (LPS) at a dose of 15 mg/kg. The miR-223 antagomir NC group and miR-223 antagomir group received consecutive tail vein injections of miR-223 antagomir NC and miR-223 antagomir, respectively, for 3 days before modeling. Reverse transcription-polymerase chain reaction (RT-PCR) was used to study the expression of miR-223 in the myocardial tissue of mice in different time point groups of the SCM model. Hematoxylin-eosin (HE) staining was performed to observe the myocardial pathological changes in the Normal group, SCM group, miR-223 antagomir NC group, and miR-223 antagomir group. Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of cTnI, BNP, CK-MB, IL-6, IL-1β, and TNF-α in mouse serum in the Normal group, SCM group, miR-223 antagomir NC group, and miR-223 antagomir group, and correlation analysis was conducted. Results: In the SCM model groups at different time points, the expression level of miR-223 in the myocardial tissue gradually increased with the extension of stimulation time. Compared to the Normal group, mice in the SCM group, miR-223 antagomir NC group, and miR-223 antagomir group exhibited varying degrees of myocardial damage. The expression levels of cardiac injury markers cTnI, BNP, CK-MB, and inflammatory factors IL-6, IL-1β, and TNF-α in serum were elevated, and the differences were statistically significant (P<0.05). When compared to the SCM group, there were no significant differences in various indicators between the miR-223 antagomir NC group (P>0.05). In the miR-223 antagomir group, the degree of pathological damage in the myocardial tissue was reduced, and the levels of cardiac injury markers cTnI, BNP, CK-MB, and inflammatory factors IL-6, IL-1β, and TNF-α decreased, with statistically significant differences (P<0.05). The results of correlation analysis showed that the expression of miR-223 in mice was positively correlated with the expression of cardiac injury markers cTnI, BNP, CK-MB, and inflammatory factors IL-6, IL-1β, and TNF-α. Conclusion: Downregulation of miR-223 expression can provide protection to the myocardium of SCM mice by alleviating the inflammatory response. |
查看全文
查看/发表评论 下载PDF阅读器 |
关闭 |
|
|
|