| 李 晨,樊 凯,刘 毅,冯向阳,杜 靖.肾上腺髓质切除对慢性间歇性缺氧大鼠的血管重塑作用[J].,2023,(23):4412-4419 |
| 肾上腺髓质切除对慢性间歇性缺氧大鼠的血管重塑作用 |
| Effect of Adrenal Medulectomy on Vascular Remodeling in Chronic Intermittent Hypoxia Rats |
| 投稿时间：2023-01-26 修订日期：2023-02-23 |
| DOI：10.13241/j.cnki.pmb.2023.23.003 |
| 中文关键词: 慢性间歇性缺氧 肾上腺髓质 血管重塑 |
| 英文关键词: Chronic intermittent hypoxia Adrenal medulla Vascular remodeling |
| 基金项目:新疆维吾尔自治区自然科学基金项目（2020D01C159）；"十四五"重点培育学科-基础医学 |
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| 中文摘要: |
| 摘要 目的：探究肾上腺髓质切除对慢性间歇性缺氧（CIH）大鼠的血管重塑作用。方法：将32只大鼠随机分为慢性间歇性空气（CIA）假手术组、CIA手术组、CIH假手术组、CIH手术组。制备CIH模型，手术组在缺氧前行肾上腺髓质切除术，假手术组行相同操作但不切除肾上腺髓质。测量尾动脉血压；ELISA法检测血清肾上腺素（E）、去甲肾上腺素（NE）含量；HE、Masson染色和荧光显微镜观察胸主动脉形态，计算胸主动脉中膜厚度、胶原容积分数和弹性纤维紊乱断裂比例；IHC观察胸主动脉α-平滑肌肌动蛋白（α-SMA）表达；TUNEL观察胸主动脉细胞凋亡率；WB检测胸主动脉天冬氨酸半胱氨酸酶-3（Caspase-3）蛋白表达。结果：与CIA假手术组比较，CIH假手术组E、NE、尾动脉收缩压、舒张压、胸主动脉中膜厚度、胶原容积分数、弹性纤维紊乱断裂比例、α-SMA水平、细胞凋亡率、Caspase-3表达升高（P＜0.05）；与CIH假手术组比较，CIH手术组E、NE、尾动脉收缩压、舒张压、胸主动脉中膜厚度、胶原容积分数、弹性纤维紊乱断裂比例、α-SMA水平、细胞凋亡率、Caspase-3表达降低（P＜0.05）。结论：切除肾上腺髓质可改善由慢性间歇性缺氧引起的大鼠胸主动脉重塑。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of adrenomedulectomy on vascular remodeling in rats with chronic intermittent hypoxia (CIH). Methods: Thirty-two rats were randomly divided into chronic intermittent air (CIA) sham-operated group, CIA operated group, CIH sham-operated group and CIH operated group. The CIH model was established. The operation group underwent adrenomedulectomy before hypoxia, and the sham-operation group underwent the same operation without adrenomedulectomy. Blood pressure of tail artery was measured. The levels of epinephrine (E) and norepinephrine (NE) in serum were detected by ELISA. HE and Masson staining and fluorescence microscopy were used to observe the morphology of thoracic aorta, and the thickness of thoracic aorta media, collagen volume fraction and the proportion of elastic fiber disorder and rupture were calculated. The expression of α-smooth muscle actin (α-SMA) in thoracic aorta was observed by IHC. TUNEL was used to observe the apoptosis rate of thoracic aorta cells. Western blot was used to detect the expression of Caspase-3 protein in thoracic aorta. Results: Compared with the CIA sham-operation group, the E, NE, tail artery systolic blood pressure, diastolic blood pressure, thoracic aortic media thickness, collagen volume fraction, proportion of elastic fiber disorder and rupture, α-SMA level, apoptosis rate and Caspase-3 expression were significantly increased in the CIH sham-operation group (P<0.05). Compared with CIH sham-operation group, the E, NE, systolic blood pressure and diastolic blood pressure of tail artery, thoracic aortic media thickness, collagen volume fraction, proportion of elastic fiber disorder and rupture, α-SMA level, apoptosis rate and Caspase-3 expression were significantly decreased in CIH operation group (P<0.05). Conclusion: Adrenomedulectomy can improve thoracic aorta remodeling induced by chronic intermittent hypoxia in rats. |
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