| 陈耀华,豆亚伟,周理乾,孙孟锟,樊国峰.黄芪多糖基于PI3K/Akt通路对食管癌大鼠抑瘤作用及免疫功能的影响[J].,2023,(16):3032-3036 |
| 黄芪多糖基于PI3K/Akt通路对食管癌大鼠抑瘤作用及免疫功能的影响 |
| Influences of Astragalus Polysacharide on Anti-tumor Effect and Immune Function of Esophageal Carcinoma Rats through PI3K/Akt Signal Pathway |
| 投稿时间：2023-03-07 修订日期：2023-03-31 |
| DOI：10.13241/j.cnki.pmb.2023.16.006 |
| 中文关键词: 黄芪多糖 食管癌 抑瘤作用 免疫功能 PI3K/Akt信号通路 |
| 英文关键词: Astragalus polysacharide Esophageal carcinoma Anti-tumor effect Immune function PI3K/Akt signal pathway |
| 基金项目:陕西省重点研发计划项目（2023-YBSF-021） |
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| 中文摘要: |
| 摘要 目的：探究黄芪多糖（APS）对食管癌（EPC）模型大鼠的抑瘤作用、免疫功能及磷脂酰肌醇-3-激酶（PI3K）/蛋白激酶B（Akt）信号通路的影响。方法：选取6周龄SPF级健康SD大鼠50只，随机分为模型组(M组)、顺铂组（S组）、黄芪多糖低剂量组（APSL）、黄芪多糖中剂量组（APSM）、黄芪多糖高剂量组（APSH），每组10只。通过移植人食管癌Eca109细胞建立EPC模型，分别给予3 mg/kg顺铂和100、200、400 mg/kg的APS干预。测量各组大鼠肿瘤体积和肿瘤质量，计算肿瘤生长抑制率、胸腺指数和脾指数，HE染色观察肿瘤组织形态学，采用流式细胞仪检测外周血CD3+、CD4+、CD8+T淋巴细胞群，Western blot测定各组肿瘤组织中PI3K和Akt蛋白的相对表达。结果：经干预后，S组和各APS组大鼠肿瘤体积和肿瘤质量均显著小于M组（P＜0.05）；S组和APSH组肿瘤体积和肿瘤质量相显著小于APSM和APSL组（P＜0.05）；APSM组大鼠肿瘤体积和肿瘤质量显著小于APSL组（P＜0.05）；APSH、APSM和APSL组的抑瘤率分别为45.59%、32.35%和17.65%。S组大鼠的胸腺指数和脾指数均显著低于M组（P＜0.05）；各APS组大鼠的胸腺指数和脾指数均显著高于S组（P＜0.05）；APSH和APSM组大鼠胸腺指数和脾指数均显著高于M组（P＜0.05）。各APS组大鼠CD3+、CD4+和CD4+/CD8+均显著高于S组和M组，而CD8+均显著低于S组和M组（P＜0.05）；APSH、APSM和APSL组之间两两相比亦均有统计学差异（P＜0.05）。S组和各APS组PI3K/Akt信号通路蛋白的相对表达均显著低于M组（P＜0.05）；APSH、APSM和APSL组之间两两相比亦均有统计学差异（P＜0.05）。结论：APS可通过调控PI3K/Akt 信号通路和改善免疫功能，发挥对EPC的抑制作用，为临床治疗EPC提供了新的思路和理论依据。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the influences of astragalus polysacharide(APS) on anti-tumor effect and immune function of esophageal carcinoma(EPC) rats through phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) signal pathway. Methods: 50 healthy 6-week-old SPF grade SD rats were randomly divided into model group(group M), cisplatin group(group S), APS low dosage group(group APSL), APS middle dosage group(group APSM) and APS high dosage group(group APSH), with 10 rats in each group. EPC model rats were established by transplantation of Eca109 cells, and 3mg/kg cisplatin and 100, 200, 400 mg/kg APS were given to each rat. Tumor volume and tumor mass were detected, tumor growth inhibition rate, thymus index and spleen index were calculated, histomorphology was observed by HE staining, CD3+, CD4+, CD8+T lymphocyte population were detected by flow cytometer, the relative expressions of PI3K and Akt protein were detected by Western blot. Results: After intervention, tumor volume and tumor mass of group S and group APS were all lower than group M(P＜0.05); the tumor volume and tumor mass of group S and group APSH were all lower than group APSM and group APSL(P＜0.05); the tumor growth inhibition rate of each APS group were 45.59%, 32.35% and 17.65%. The thymus index and spleen index of group S were lower than group M(P＜0.05); the thymus index and spleen index of each APS group were all higher than group S(P＜0.05); while group APSH and APSM were higher than group M(P＜0.05). The CD3+, CD4+ and CD4+/CD8+ of each APS group were higher than group S and group M, while the CD8+ was lower(P＜0.05); group APSH, APSM and APSL also had statistical differences(P＜0.05). The relative expressions of PI3K/Akt signal pathway protein of group S and each APS group were lower than group M(P＜0.05); group APSH, APSM and APSL also had statistical differences(P＜0.05). Conclusion: APS exerts inhibition effect on EPC via regulation of PI3K/Akt signal pathway and immune function, which can provide new thought and theoretical basis for clinical treatment of EPC. |
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