| 王闻天,罗汝茜,李 慧,张 慧,郝 丽.重度牙周炎对阿尔茨海默病模型大鼠肠道菌群及神经炎症因子表达的影响[J].,2023,(11):2040-2045 |
| 重度牙周炎对阿尔茨海默病模型大鼠肠道菌群及神经炎症因子表达的影响 |
| Impacts of Severe Periodontitis on Intestinal Flora and Expression of Neuroinflammatory Factors in Rats with Alzheimer's Disease Model |
| 投稿时间：2023-02-25 修订日期：2023-03-20 |
| DOI：10.13241/j.cnki.pmb.2023.11.008 |
| 中文关键词: 重度牙周炎 阿尔茨海默病 大鼠 肠道菌群 神经炎症因子 |
| 英文关键词: Severe periodontitis Alzheimer's disease Rat Intestinal flora Neuroinflammatory factor |
| 基金项目:北京市住院医师规范化培训质量提高项目实施方案探索项目（住培2022021） |
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| 中文摘要: |
| 摘要 目的：探讨重度牙周炎对阿尔茨海默病（AD）模型大鼠肠道菌群及神经炎症因子表达的影响。方法：将SD大鼠随机平分为空白对照组（NC组）、牙周炎组（P组）、AD组、P+AD组，每组10只大鼠。NC组不做任何处理，P组和AD组大鼠分别构建重度牙周炎模型和AD模型，P+AD组同时构建重度牙周炎模型和AD模型。8周后，采用Morris水迷宫实验观察大鼠的空间学习和记忆能力；酶联免疫吸附（ELISA）法检测血清炎性因子：白介素-1β（IL-1β）、肿瘤坏死因子α（TNF-α）、白介素10（IL-10）水平；苏木精-伊红（HE）染色检测海马组织病理变化；粪便进行短链脂肪酸（SCFAs）测序及16S rRNA基因测序。结果：NC组和P组大鼠神经元排列整齐且紧凑，没有明显的细胞空泡和坏死，AD组和P+AD组海马组织中观察到神经元变性和坏死，海马区锥体细胞排列松散，空泡化。与NC组相比较，AD组逃逸潜伏期、IL-1β、TNF-α水平、拟杆菌门、变形菌门、链球菌属、普雷沃菌属、大肠杆菌志贺氏菌属的相对丰度显著增加（P<0.05），停滞时间、穿越平台次数、IL-10水平、乙酸盐、丙酸盐、丁酸盐的含量及Shannon、Simpson、Chao1指数、厚壁菌门、乳酸杆菌属的相对丰度显著减少（P<0.05），而NC组与P组以上指标对比差异不显著（P＞0.05）；与AD组、P组相比，P+AD组逃逸潜伏期、IL-1β、TNF-α水平、拟杆菌门、变形菌门、链球菌属、普雷沃菌属、大肠杆菌志贺氏菌属的相对丰度显著增加（P<0.05），停滞时间、穿越平台次数、IL-10水平、乙酸盐、丙酸盐、丁酸盐的含量及Shannon、Simpson、Chao1指数、厚壁菌门、乳酸杆菌属的相对丰度显著减少（P<0.05）。结论：重度牙周炎可促进AD大鼠肠道菌群紊乱及神经炎症因子表达。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the impacts of severe periodontitis on intestinal flora and expression of neuroinflammatory factors in rats with Alzheimer's disease (AD) model. Methods: SD rats were randomly grouped into blank control group (NC group), periodontitis group (P group), AD group and P+AD group, with 10 rats in each group. The NC group did not do any treatment. The rats in P group and AD group were respectively constructed with severe periodontitis model and AD model, while the rats in P+AD group were also constructed with severe periodontitis model and AD model. After 8 weeks, Morris water maze experiment was used to observe the spatial learning and memory ability of rats. The level of serum inflammatory factors including interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) were determined by enzyme-linked immunosorbent assay (ELISA) method. Hematoxylin-eosin (HE) staining was used to detect the pathological changes of hippocampus. Short chain fatty acids (SCFAs) and 16S rRNA gene were sequenced in feces. Results: The neurons in the NC group and P group were arranged neatly and compactly, without obvious cell vacuoles and necrosis. Neuronal degeneration and necrosis were observed in the hippocampus in the AD group and P+AD group, and the pyramidal cells in the hippocampus were arranged loosely and vacuolized. Compared with NC group, the escape latency, the levels of IL-1β, TNF-α, the relative abundances of Bacteroidetes, Proteobacteria, Streptococcus, Prevotella, Escherichia-Shigella in the AD group increased obviously(P<0.05), Stagnation time, times of platform crossing, the level of IL-10, the content of acetate, propionate, butyrate, the Shannon, Simpson, Chao1 indexes, relative abundances of Firmicutes and Lactobacillus decreased obviously (P<0.05), there was no significant difference in the above indexes between NC group and P group(P>0.05). Compared with AD group and P group, the escape latency, the levels of IL-1β, TNF-α, the relative abundances of Bacteroidetes, Proteobacteria, Streptococcus, Prevotella, Escherichia- Shigella in the P+AD group increased obviously(P<0.05), Stagnation time, times of platform crossing, the level of IL-10, the content of acetate, propionate, butyrate, the Shannon, Simpson, Chao1 indexes, relative abundances of Firmicutes and Lactobacillus decreased obviously(P<0.05). Conclusion: Severe periodontitis can promote the disorder of intestinal flora and the expression of neuroinflammatory factors in rats with AD. |
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